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Bats, the second-largest mammalian order, are widely distributed and provide crucial ecosystem services. Their reproductive biology comprises noteworthy characteristics, such as long-term sperm storage, delayed implantation or even delayed fetal development. The understanding of these mechanisms remains limited. Research in reproductive biology may become crucial for the conservation of endangered bat species. Indeed, the IUCN Red List of Threatened Species currently lists 76 bat species as endangered or critically endangered, for which conservation measures should be taken. The development of assisted reproductive technologies (ART) in bats would thus open new perspectives. In this context we here describe the first successful cryopreservation of bat sperm in the Seba's short-tailed bat (Carollia perspicillata). Epididymal sperm of 30 males was pooled in 6 sample sets and diluted using four different cryo-diluents: Canifreeze, modified Canifreeze, BotuCrio and Test egg yolk extender. Glycerol and a mixture of glycerol and methylformamide were used as cryoprotectants. Best post-thaw results were achieved when sperm was diluted in Test egg yolk extender containing a mixture of glycerol and methylformamide as cryoprotectant. Post-thaw sperm motility, progressive motility and acrosome integrity of 42.0 ± 3.4%, 28.3 ± 6.3% and 87.7 ± 1.7%, respectively were superior to all other variants tested. It remains to be determined if the post-thaw quality of cryopreserved bat spermatozoa achieved in this study is sufficient for the use in artificial inseminations and produces acceptable pregnancy rates. Successful cryopreservation of spermatozoa in C. perspicillata may serve as the first model to establish gamete rescue programs and develop ART in other, critically endangered bat species.
This article was published in the following journal.
Sperm vitrification (V) is a method for cryopreservation, without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen (LN25).
The main aim of the present review is to present the opportunities and challenges associated with the application of cryopreserved sperm, which may improve the breeding of salmonid fishes. Cryopreserv...
Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are...
The objective is to systematically review and synthesize the literature on the efficacy with two different cryopreservation methods used for human spermatozoa and evaluate whether vitrification protoc...
Little is known of the genetic variability in semen quality traits in cattle and their inter-relationships. The objective of the present study was to estimate genetic parameters for a range of semen q...
Sperm freezing has been employed for decades for male fertility preservation in cases of foreseeable or unexpected loss of fertility to guarantee future paternity, and also as a complement...
Cryopreservation is the storage of biological material at subzero temperatures at which biochemical processes of cell metabolism and the biochemical reactions that lead to cell death are s...
The objective of this study is to evaluate the effect of in-vitro myoinositol supplementation of human sperm on the outcome of cryopreservation.
Despite the known adverse effects of specific cancer treatments on fertility, only 18-26% of at-risk adolescents and young men cryopreserve sperm prior to cancer treatment in the US:These ...
Background: Due to the remarkable improvement in treatments these last decades, long term survival can be expected in more than 80% of childhood cancer patients. Unfortunately, cancer tre...
The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
The structural and functional changes by which SPERMATOZOA become capable of oocyte FERTILIZATION. It normally requires exposing the sperm to the female genital tract for a period of time to bring about increased SPERM MOTILITY and the ACROSOME REACTION before fertilization in the FALLOPIAN TUBES can take place.
Standard antiretroviral therapy (ART) consists of the combination of at least three antiretroviral (ARV) drugs to maximally suppress the HIV virus and stop the progression of HIV disease. Huge reductions have been seen in rates of death and suffering whe...
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