Programming Target-initiated Bifunctional DNAzyme Nanodevice for Sensitive Ratiometric Electrochemical Biosensing.

08:00 EDT 1st April 2019 | BioPortfolio

Summary of "Programming Target-initiated Bifunctional DNAzyme Nanodevice for Sensitive Ratiometric Electrochemical Biosensing."

Here, a novel bifunctional DNAzyme nanodevice (BFDN) with two detection paths toward the same target was intelligently designed and applied to construct a novel ratiometric electrochemical biosensor for highly reliable and sensitive mercury ion (Hg2+) detection. In the presence of target Hg2+, T-Hg2+-T pair could actuate the preassembled DNA four-branched nanostructure (DNA-4B) without cleavage capability transform into the BFDN with strong cleavage capability for triggering two synchronous Hg2+ detection paths, including a "signal-off" path 1 that consisted of a cascade DNAzyme cleavage reaction to dramatically decrease the ferrocene (Fc) response and a "signal-on" path 2 that accomplished the capture of significant amounts of methylene blue (MB) on the electrode surface under the assistant of DNAzyme2 (D2) in BFDN. This strategy not only effectively avoided the false positive signal compared with traditional single path, but also proposed a new ratiometric method to successfully circumvent the deficiency existed in previous ratiometric electrochemical biosensors. As a result, the reliable and sensitive Hg2+ detection was achieved in the range from 0.1 pM to 200 nM with a detection limit of 23 fM. Above all, the assembly of the BFDN here is ingeniously coupled with amplification strategy, paving a promising avenue to promote the performances of simple multi-functional DNA nanomachines and facilitate the corresponding development of DNA nanomachines in biosensor platform.


Journal Details

This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882


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