Enhanced Sample Handling for Analytical Ultracentrifugation With 3D-Printed Centerpieces.

08:00 EDT 1st April 2019 | BioPortfolio

Summary of "Enhanced Sample Handling for Analytical Ultracentrifugation With 3D-Printed Centerpieces."

The centerpiece of the sample cell assembly in analytical ultracentrifugation holds the sample solution between windows, sealed against high vacuum, and is shaped such that macromolecular migration in centrifugal fields exceeding 200,000g can proceed undisturbed by walls or convection while concentration profiles are imaged with optical detection systems aligned perpendicular to the plane of rotation. We have recently shown that 3D printing using various materials allows inexpensive and rapid manufacturing of centerpieces. In the present work, we expand this endeavor to examine the accuracy of the measured sedimentation process, as well as short-term durability of the centerpieces. We find that 3D-printed centerpieces can be used many times, and can provide data equivalent in quality to commonly used commercial epoxy resin centerpiec-es. Furthermore, 3D printing enables novel designs adapted to particular experimental objectives, because they offer unique opportunities, for example, to create well-defined curved surfaces, narrow channels, and embossed features. We present examples of centerpiece designs exploiting these capabilities for improved AUC experiments. This includes narrow sector centerpieces that substantially reduce the required sample volume while maintaining the standard optical pathlength; thin centerpieces with integrated window holders to provide very short optical pathlengths that reduce optical aberrations at high macromolecular concentrations; long-column centerpieces that increase the observable distance of macromolecular migra-tion for higher-precision sedimentation coefficients; and three-sector centerpieces that allow doubling the number of sam-ples in a single run while reducing the sample volumes. We find each of these designs allows unimpeded macromolecular sedimentation and can provide high-quality sedimentation data.


Journal Details

This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882


DeepDyve research library

PubMed Articles [11763 Associated PubMed Articles listed on BioPortfolio]

Analytical ultracentrifugation (AUC): a seminal tool offering multiple solutions.

Analytical ultracentrifugation (AUC) remains a highly versatile and widely applicable tool for the analysis of macromolecules and their interactions. The current state-of-the-art was demonstrated at a...

A 3D printed fluidic device for measuring pharmaceuticals in biological fluids.

Multimaterial 3D printing provides a unique capability for the creation of highly complex integrated devices where complementary functionality is realized using differences in material properties. Usi...

Analytical aspects of printed oral dosage forms.

Printing technologies, both 2D and 3D, have gained considerable interest during the last years for manufacturing of personalized dosage forms, tailored to each patient. Here we review the research wor...

Enumeration of rare cells in whole blood by signal ion emission reactive release amplification (SIERRA) with same-sample RNA analysis.

Herein is presented a platform capable of detecting less than 30 cells from a whole blood sample by size-exclusion filtration, microfluidic sample handling, and mass spectrometric detection through si...

Automated 4-Sample Protein Immunoassays using 3D-Printed Microfluidics.

Low cost, miniaturized assay platforms that work with small sample volumes, high sensitivity and rapid detection will have high value in future biomolecular diagnostics. Herein we report an automated,...

Clinical Trials [3696 Associated Clinical Trials listed on BioPortfolio]

Pre-analytical Sample Handling Conditions and Their Effects on the Human Urine Metabolome

This study evaluates how urine handling conditions can affect the concentrations of ~1,000 urine metabolites and attempts to quantify the degree of attenuation and/or potential bias in epi...

Integrating Palliative Care for Patients With Idiopathic Pulmonary Fibrosis and Their Caregivers

Patients with Idiopathic Pulmonary Fibrosis (IPF) and their caregivers will be randomized to receive this intervention or usual care. The intervention will include information about the di...

Accuracy of 3D Printed Maxillary Complete Dentures

Conventional complete dentures have been used for almost a century as a treatment choice for edentulous patients, despite of their inaccuracy & dimensional changes during manufacturing. Re...

Regulation of Metabolism and Ion Handling

This study investigates the relation between ion-handling (K+) and fatigue development during exercise. Before and after a 5-week period of high intensity interval training, performance an...

Serum Sample Collection to Determine Analytical Performance Characteristics of the ADVIA Centaur PAPPA and BhCG Assays

Blood samples collected will be shipped to the sponsor's laboratory to establish analytical performance characteristics of the ADVIA Centaur® Pregnancy Associated Plasma Protein A (PAPP-A...

Medical and Biotech [MESH] Definitions

Methods utilizing the principles of MICROFLUIDICS for sample handling, reagent mixing, and separation and detection of specific components in fluids.

The analysis of a chemical substance by inserting a sample into a carrier stream of reagent using a sample injection valve that propels the sample downstream where mixing occurs in a coiled tube, then passes into a flow-through detector and a recorder or other data handling device.

A solventless sample preparation method, invented in 1989, that uses a fused silica fiber which is coated with a stationary phase. It is used for sample cleanup before using other analytical methods.

Works consisting of advertisements separately printed and distributed by a publisher to describe and solicit orders for a recent or forthcoming publication. In the case of books, they may include sample pages. (From: ALA Glossary of Library and Information Science, 1983)

Laboratory processes prior to specimen analysis. These processes include study design, compliance of the subjects investigated, compliance in adherence to protocols, choice of specimens utilized and sample collection.

Quick Search


DeepDyve research library

Searches Linking to this Article