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Enhanced Sample Handling for Analytical Ultracentrifugation With 3D-Printed Centerpieces.

08:00 EDT 1st April 2019 | BioPortfolio

Summary of "Enhanced Sample Handling for Analytical Ultracentrifugation With 3D-Printed Centerpieces."

The centerpiece of the sample cell assembly in analytical ultracentrifugation holds the sample solution between windows, sealed against high vacuum, and is shaped such that macromolecular migration in centrifugal fields exceeding 200,000g can proceed undisturbed by walls or convection while concentration profiles are imaged with optical detection systems aligned perpendicular to the plane of rotation. We have recently shown that 3D printing using various materials allows inexpensive and rapid manufacturing of centerpieces. In the present work, we expand this endeavor to examine the accuracy of the measured sedimentation process, as well as short-term durability of the centerpieces. We find that 3D-printed centerpieces can be used many times, and can provide data equivalent in quality to commonly used commercial epoxy resin centerpiec-es. Furthermore, 3D printing enables novel designs adapted to particular experimental objectives, because they offer unique opportunities, for example, to create well-defined curved surfaces, narrow channels, and embossed features. We present examples of centerpiece designs exploiting these capabilities for improved AUC experiments. This includes narrow sector centerpieces that substantially reduce the required sample volume while maintaining the standard optical pathlength; thin centerpieces with integrated window holders to provide very short optical pathlengths that reduce optical aberrations at high macromolecular concentrations; long-column centerpieces that increase the observable distance of macromolecular migra-tion for higher-precision sedimentation coefficients; and three-sector centerpieces that allow doubling the number of sam-ples in a single run while reducing the sample volumes. We find each of these designs allows unimpeded macromolecular sedimentation and can provide high-quality sedimentation data.

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This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882
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