Advertisement

Topics

Rapid and Sensitive Detection of in Chickens Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick.

08:00 EDT 28th March 2019 | BioPortfolio

Summary of "Rapid and Sensitive Detection of in Chickens Using Loop-Mediated Isothermal Amplification Combined with a Lateral Flow Dipstick."

Salmonellosis is a highly contagious bacterial disease that threatens both human and poultry health. Tests that can detect in the field are urgently required to facilitate disease control and for epidemiological investigations. Here, we combined loop-mediated isothermal amplification (LAMP) with a chromatographic lateral flow dipstick (LFD) to rapidly and accurately detect . LAMP primers were designed to target the gene. LAMP conditions were optimized by adjusting the ratio of inner to outer primers, MgSO concentration, dNTP mix concentration, amplification temperature, and amplification time. We evaluated the specificity of our novel LAMP-LFD method using six species and six related non- strains. All six of the strains, but none of the non- strains, were amplified. LAMP-LFD was sensitive enough to detect concentrations of subsp. serovar Pullorum genomic DNA as low as 89 fg/µl, which is 1,000 times more sensitive than conventional PCR. When artificially contaminated feed samples were analyzed, LAMP-LFD was also more sensitive than PCR. Finally, LAMP-LFD gave no false positives across 350 chicken anal swabs. Therefore, our novel LAMP-LFD assay was highly sensitive, specific, convenient, and fast, making it a valuable tool for the early diagnosis and monitoring of infection in chickens.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of microbiology and biotechnology
ISSN: 1738-8872
Pages: 454-464

Links

DeepDyve research library

PubMed Articles [17512 Associated PubMed Articles listed on BioPortfolio]

Rapid Visual Detection of Getah Virus Using a Loop-Mediated Isothermal Amplification Method.

Getah virus (GETV) is a mosquito-borne alphavirus that is considered to be an emerging pathogen. To date, reverse transcription loop-mediated isothermal amplification (RT-LAMP) has not been used to de...

Rapid and sensitive detection of cucumber mosaic virus by reverse transcription loop-mediated isothermal amplification.

Development of a loop-mediated isothermal amplification assay for specific detection of all known subspecies of Clavibacter michiganensis.

Clavibacter michiganensis is an important bacterial plant pathogen that causes vast destruction to agriculturally important crops worldwide. Early detection is critical to evaluate disease progression...

Probe-free label system for rapid detection of Cronobacter genus in powdered infant formula.

Cronobacter species previously known as Enterobacter sakazakii poses high risks to neonates and infants. In this work a rapid detection method was developed which combined loop-mediated isothermal amp...

Point-of-care rapid detection of Vibrio parahaemolyticus in seafood using loop-mediated isothermal amplification and graphene-based screen-printed electrochemical sensor.

Vibrio parahaemolyticus is one of the most important foodborne pathogens that cause various life-threatening diseases in human and animals. Here, we present a rapid detection platform for V. parahaemo...

Clinical Trials [5443 Associated Clinical Trials listed on BioPortfolio]

Clinical Performance of the HRP2 HS-RDT for Malaria Diagnosis in Pregnant Women

This is a cross-sectional and multicentre clinical trial to study the performance of the Histidine Rich Protein 2 (HRP2) highly sensitive rapid diagnostic test (HS-RDT) for the detection o...

Evaluation of the Alere i Influenza A&B Nucleic Acid Amplification Versus Xpert Flu/RSV

The rapid and accurate detection of influenza virus in respiratory specimens is required for optimal management of patients with acute respiratory infections. Because of the variability of...

Discrimination of P and T Waves With Linq and Holter

Currently loop recorders diagnose arrhythmias and send automatic alerts to physicians based on R to R relationships only. We believe arrhythmia diagnostics could be improved if accurate de...

Validation of RealAmp Method for the Diagnosis of Malaria in Endemic Areas of Brazil

Worldwide, approximately 2 billion people live in areas at risk for malaria with morbidity surpassing 250 million cases, with approximately 800,000 deaths, per year. Of the four species of...

Using Eggshell Calcium to Mitigate Fluorosis in Ethiopia

Mothers and young children in rural Ethiopia lack sufficient nutrition, and a compounding factor is presence of high levels of fluoride (> 5-10 mg/day) affecting body functions. Interventi...

Medical and Biotech [MESH] Definitions

Sensitive method for detection of bacterial endotoxins and endotoxin-like substances that depends on the in vitro gelation of Limulus amebocyte lysate (LAL), prepared from the circulating blood (amebocytes) of the horseshoe crab, by the endotoxin or related compound. Used for detection of endotoxin in body fluids and parenteral pharmaceuticals.

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

A mechanism of communication with a physiological system for homeostasis, adaptation, etc. Physiological feedback is mediated through extensive feedback mechanisms that use physiological cues as feedback loop signals to control other systems.

Still images produced from radiation-sensitive materials (sensitive to light, electron beams, or nuclear radiation), generally by means of the chemical action of light on a sensitive film, paper, glass, or metal. Photographs may be positive or negative, opaque or transparent.

Ascertaining of deception through detection of emotional disturbance as manifested by changes in physiologic processes usually using a polygraph.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Food
Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...


Searches Linking to this Article