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Heat-resistant microbial hosts are required for bioprocess development using high cell density cultivations at the industrial scale. We report that the thermotolerance of can be enhanced by overexpressing , which was known to encode a hypothetical protein of unknown function. In the wild-type BL21(DE3), transcription level increased over five-fold when temperature was increased from 37°C to either 42°C or 46°C. To study the function of , a deletion strain and an overexpression strain were constructed. At 46°C, in comparison to the wild type, the -deletion reduced cell growth half-fold, and the -overexpression promoted cell growth over two-fold. The growth enhancement by -overexpression was much more pronounced at 46°C than 37°C. The -overexpression was also effective in other strains of MG1655, W3110, DH10B, and BW25113. These findings reveal that gene plays an important role in enduring high-temperature stress, and that -overexpression can be a prospective strategy to develop thermotolerant microbial hosts.
This article was published in the following journal.
Name: Journal of microbiology and biotechnology
Heat stress is a severe environmental stress that affects plant growth and reduces yield. Bax inhibitor-1 (BI-1) is a cytoprotective protein that is involved in the response to biotic and abiotic stre...
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KEY MESSAGE: (1) The fes1a bag6 double mutant shows an increased short term thermotolerance compared to fes1a. BAG6 is a suppressor of Fes1A; (2) IQ motif is essential to effective performance of BAG6...
Thermotolerance is a phenomenon in which cells become resistant to stress by prior exposure to heat shock, and its development is associated with the induction of heat shock proteins (Hsps), including...
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The ability of an organism to reduce susceptibility to heat shock, and adapt to HOT TEMPERATURE.
A potent epoxide hydrase and aryl hydrocarbon hydroxylase inhibitor. It enhances the tumor-initiating ability of certain carcinogens.
A physiologically active metabolite of VITAMIN D. The compound is involved in the regulation of calcium metabolism, alkaline phosphatase activity, and enhances the calcemic effect of CALCITRIOL.
An indolocarbazole that is a potent PROTEIN KINASE C inhibitor which enhances cAMP-mediated responses in human neuroblastoma cells. (Biochem Biophys Res Commun 1995;214(3):1114-20)
A cytokine which resembles IL-1 structurally and IL-12 functionally. It enhances the cytotoxic activity of NK CELLS and CYTOTOXIC T-LYMPHOCYTES, and appears to play a role both as neuroimmunomodulator and in the induction of mucosal immunity.
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Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...