Advertisement

Topics

Using resonance synchronous spectroscopy to characterize the reactivity and electrophilicity of biologically relevant sulfane sulfur.

08:00 EDT 26th March 2019 | BioPortfolio

Summary of "Using resonance synchronous spectroscopy to characterize the reactivity and electrophilicity of biologically relevant sulfane sulfur."

Sulfane sulfur is common inside cells, playing both regulatory and antioxidant roles. However, there are unresolved issues about its chemistry and biochemistry. We report the discovery that reactive sulfane sulfur such as polysulfides and persulfides could be detected by using resonance synchronous spectroscopy (RS). With RS, we showed that inorganic polysulfides at low concentrations were unstable with a half-life about 1 min under physiological conditions due to reacting with glutathione. The protonated form of glutathione persulfide (GSSH) was electrophilic and had RS signal. GSS was nucleophilic, prone to oxidation, but had no RS signal. Using this phenomenon, pK of GSSH was determined as 6.9. GSSH/GSS was 50-fold more reactive than HS/HS towards HO at pH 7.4, supporting reactive sulfane sulfur species like GSSH/GSS may act as antioxidants inside cells. Further, protein persulfides were shown to be in two forms: at pH 7.4 the deprotonated form (R-SS) without RS signal was not reactive toward sulfite, and the protonated form (R-SSH) in the active site of a rhodanese had RS signal and readily reacted with sulfite to produce thiosulfate. These data suggest that RS of sulfane sulfur is likely associated with its electrophilicity. Sulfane sulfur showed species-specific RS spectra and intensities at physiological pH, which may reveal the relative abundance of a reactive sulfane sulfur species inside cells.

Affiliation

Journal Details

This article was published in the following journal.

Name: Redox biology
ISSN: 2213-2317
Pages: 101179

Links

DeepDyve research library

PubMed Articles [10571 Associated PubMed Articles listed on BioPortfolio]

O NMR and N NMR chemical shifts of sterically-hindered amides: ground-state destabilization in amide electrophilicity.

The structure and spectroscopic properties of the amide bond are a topic of fundamental interest in chemistry and biology. Herein, we report 17O NMR and 15N NMR spectroscopic data for four series of s...

The contribution of 1H magnetic resonance spectroscopy to the study of excitation-inhibition in autism.

Autism spectrum disorder (ASD) affects over 1:100 of the population and costs the UK more than £32bn and the USA more than $175bn (£104bn) annually. Its core symptoms are social and communication di...

Nuclear Magnetic Resonance Spectroscopy as a Dynamical Structural Probe of Hydrogen under High Pressure.

An unambiguous crystallographic structure solution for the observed phases II-VI of high pressure hydrogen does not exist due to the failure of standard structural probes at extreme pressure. In this ...

MELAS: Monitoring treatment with Magnetic Resonance Spectroscopy.

To assess the utility of Magnetic Resonance Spectroscopy (MRS) as a biomarker of response to L-arginine in mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes (MELAS).

Magnetic resonance imaging and proton magnetic resonance spectroscopy for differentiating between enhanced gliomas and malignant lymphomas.

Although the treatment strategies for malignant lymphomas and gliomas differ, it is usually difficult to preoperatively distinguish between the two. Magnetic resonance spectroscopy (MRS) was recently ...

Clinical Trials [4228 Associated Clinical Trials listed on BioPortfolio]

Non-Invasive Magnetic Resonance Imaging (MRI) and Magnetic Resonance Spectroscopy Techniques (MRS) for Assessing Neoadjuvant Chemotherapy in Locally Advanced Breast Cancer

The goal of this clinical research study is to learn if magnetic resonance imaging (MRI) with magnetic resonance spectroscopy (MRS) can show the effects of pre-surgical chemotherapy in bre...

Using Magnetic Resonance Spectroscopy With MRI to Non-Invasively Determine Breast Cancer Extent of Disease

The purpose of this study is to evaluate whether high-resolution magnetic resonance imaging of the axilla with spectroscopy can identify nodal features suggestive of metastatic involvement...

Safety and Feasibility of Preoperative and Intraoperative Image-Guided Resection of Gliomas

This study evaluates the use of specialized magnetic resonance imaging (MRI) techniques including magnetic resonance (MR) perfusion and 2-hydroxyglutarate (2HG) spectroscopy in the surgica...

Comparing Magnetic Resonance Imaging/Spectroscopy Techniques

Magnetic resonance imaging (MRI) and magnetic resonance spectroscopy (MRS) are diagnostic tests that allow researchers to look at different chemical properties of tissue. Magnetic resonan...

Non-invasive Measurement of Cerebrovascular Reactivity After Traumatic Brain Injury

The investigators will longitudinally measure cerebrovascular reactivity (CVR) by functional near-infrared spectroscopy (fNIRS) in acute (≤3 days from injury), subacute, and chronic phas...

Medical and Biotech [MESH] Definitions

Magnetic resonance spectroscopy involving detection of changes in the resonance of carbon-13 nuclei in organic molecules.

A technique applicable to the wide variety of substances which exhibit paramagnetism because of the magnetic moments of unpaired electrons. The spectra are useful for detection and identification, for determination of electron structure, for study of interactions between molecules, and for measurement of nuclear spins and moments. (From McGraw-Hill Encyclopedia of Science and Technology, 7th edition) Electron nuclear double resonance (ENDOR) spectroscopy is a variant of the technique which can give enhanced resolution. Electron spin resonance analysis can now be used in vivo, including imaging applications such as MAGNETIC RESONANCE IMAGING.

Spectroscopy technique which measures changes in organic compounds by tracking the spectral energy of absorption of HYDROGEN atoms.

Spectroscopic method of measuring the magnetic moment of elementary particles such as atomic nuclei, protons or electrons. It is employed in clinical applications such as NMR Tomography (MAGNETIC RESONANCE IMAGING).

NMR spectroscopy on small- to medium-size biological macromolecules. This is often used for structural investigation of proteins and nucleic acids, and often involves more than one isotope.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Searches Linking to this Article