Advertisement

Topics

Removal of pharmaceutical' estrogenic activity of sequencing batch reactor effluents assessed in the T47D-KBluc reporter gene assay.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "Removal of pharmaceutical' estrogenic activity of sequencing batch reactor effluents assessed in the T47D-KBluc reporter gene assay."

Various water treatment processes may be ineffective to remove pharmaceutical compounds (PhCs) and their by-products, leading to endocrine-disruptive activity that might be detrimental to wildlife and human health. This study investigated the degradation of carbamazepine (CBZ), diclofenac (DCF), ibuprofen (IBP), and their intermediates, as well as estrogenic activity that is not effectively removed by conventional methods. A consortium of isolated South African indigenous fungi A. niger, M. circinelloides, T. polyzona, T. longibrachiatum and R. microsporus, was used in a sequencing batch reactor (SBR) to remove PhCs, their intermediates and strongly reduce their estrogenic activity. The fungal ligninolytic enzymatic activity was determined for laccase (Lac), manganese peroxidase (MnP) and lignin peroxidase (LiP) using a spectrophotometric method. The biodegradation of PhCs and their intermediates was monitored by SPE-UPLC/MS. The in vitro estrogenic activity was assessed in the T47D-KBluc reporter gene assay. Lac, MnP and LiP production appeared to be biomass growth dependent. During a lag phase of growth, a constant biomass of about 122.04 mg/100 mL was recorded with average enzymatic activity around 63.62 U/L for Lac, 151.91 U/L for MnP and 42.12 U/L for LiP. The exponential growth phase from day 7 to day 17, was characterised by a biomass increase of 124.46 units, and an increase in enzymatic activity of 9.91 units for Lac, 99.03 units for MnP and 44.24 units for LiP. These enzymes played an important synergistic role in PhCs degradation in the cytochrome P450 system. A decrease of 13.89%, 29.7% and 16.15% in PhC concentrations was observed for CBZ, DCF and IBP, respectively, and their intermediates were identified within 4 h of incubation. The removal efficiency achieved after 24 h in the SBR was about 89.77%, 95.8% and 91.41% for CBZ, DCF and IBP, respectively. The estradiol equivalent (EEq) values of 1.71 ± 0.30 ng/L and 2.69 ± 0.17 ng/L were recorded at the start-up time and after 4 h, respectively. The presence of intermediates was found to induce estrogenic activity. The EEq values after 24 h incubation was found to be below the LoQ and below the LoD of the assay. None of the samples exhibited any anti-estrogenic activity. The fungal consortium inoculum was found to induce toxicity at a 0.4× concentration, as observed under a microscope. This study revealed that the use of the fungal consortium can remove the estrogenic activity of pharmaceutical metabolites, which appeared to be the most significant contributors to the endocrine-disrupting activity of the wastewater treatment plant effluents.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of environmental management
ISSN: 1095-8630
Pages: 209-218

Links

DeepDyve research library

PubMed Articles [18045 Associated PubMed Articles listed on BioPortfolio]

The impact of powdered keramsite on activated sludge and wastewater treatment in a sequencing batch reactor.

As the efficiency of biological wastewater treatment in activated sludge systems can be increased if powdered mineral materials are added, the work described here investigated the effect of powdered k...

Stability of aerobic granular sludge in a pilot scale sequencing batch reactor enhanced by granular particle size control.

Aerobic granulation was successfully achieved in a pilot scale sequencing batch reactor within 40 days. Then, stability of different particle size granules was explored according to their activity and...

Enhancement of COD, ammonia, phosphate and sulfide simultaneous removal by the anaerobic photosynthetic bacterium of Ectothiorhodospira magna in batch and sequencing batch culture.

An anaerobic photosynthetic bacterium, with chemical oxygen demand (COD), ammonia nitrogen (NH-N), total phosphorus (TP) and sulfide (S) simultaneous removal ability, strain SU6, was isolated and iden...

The microbial community structure change of an anaerobic ammonia oxidation reactor in response to decreasing temperatures.

In anaerobic ammonium oxidation (anammox) systems, temperature may regulate the activity of functional bacteria (e.g., anammox bacteria) and the composition of the microbial population, ultimately det...

Assessing the performance of a sequencing batch biofilm reactor bioaugmented with P. stutzeri strain XL-2 treating ammonium-rich wastewater.

Pseudomonas stutzeri XL-2, with the capability of heterotrophic nitrification-aerobic denitrification and biofilm-forming, was applied in a sequencing batch biofilm reactor (SBBR) for bioaugmented tre...

Clinical Trials [4948 Associated Clinical Trials listed on BioPortfolio]

Immunity and Safety of Inactivated Enterovirus 71 Vaccine Post-marketing Among Children Aged 6-35 Months in China

This study evaluates three batches consistency, immunity duration and safety of inactivated Enterovirus 71(EV-A71) vaccine(vero cell) post-marketing among children aged 6-35months in China...

A Study to Evaluate the Immunogenicity, Safety and Tolerability of Ad26.ZEBOV and MVA‐BN‐Filo in Healthy Adult Participants

The purpose of this study is to demonstrate that 3 different batches of Ad26.ZEBOV as prime and a single batch of MVA‐BN‐Filo as boost at a 56 day interval induce an equivalent humoral ...

Pharmacokinetic Pilot Study on Budesonide/Formoterol Device-metered Dry Powder Inhaler

The aim of this pilot study is to assess the methodology, practical arrangements and pulmonary absorption with charcoal blockage for the future bioequivalence studies. In addition, PK of b...

The Impact of Additional Oral Preparation on the Quality of Bowel Preparation for Colonoscopy

Although adequate bowel preparation is essential for successful colonoscopy, the 23% of patients had shown inadequate bowel preparation. Inadequate bowel preparation may results in incompl...

Are Cognition and Sleep Affected by Exposure to Carbon Dioxide and Bio Effluents During Sleep?

The aim of the project is to examine whether children's sleep and cognition are affected by exposure to CO2 and other bio effluents during sleep. The participants of the study are 36 child...

Medical and Biotech [MESH] Definitions

Liquid, solid, or gaseous waste resulting from mining of radioactive ore, production of reactor fuel materials, reactor operation, processing of irradiated reactor fuels, and related operations, and from use of radioactive materials in research, industry, and medicine. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

RESEARCH activity focused on pharmaceutical products and services.

A quantitative prediction of the biological, ecotoxicological or pharmaceutical activity of a molecule. It is based upon structure and activity information gathered from a series of similar compounds.

A synthetic steroid with estrogenic activity.

A daidzein derivative occurring naturally in forage crops which has some estrogenic activity.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

DNA sequencing
DNA sequencing is the process of determining the precise order of nucleotides within a DNA molecule. During DNA sequencing, the bases of a small fragment of DNA are sequentially identified from signals emitted as each fragment is re-synthesized from a ...

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Assays
An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...


Searches Linking to this Article