Synthetic Hemoglobin-Based Oxygen Carriers are an Acceptable Alternative for Packed Red Blood Cells in Normothermic Kidney Perfusion.

08:00 EDT 2nd April 2019 | BioPortfolio

Summary of "Synthetic Hemoglobin-Based Oxygen Carriers are an Acceptable Alternative for Packed Red Blood Cells in Normothermic Kidney Perfusion."

Normothermic Machine Perfusion presents a novel platform for per-transplant assessment and reconditioning of kidney grafts. Maintaining the metabolic activity of a preserved graft at physiologic levels requires an adequate oxygen supply, typically delivered by crystalloid solutions supplemented with red blood cells. In this study, we explored the feasibility of using a synthetic hemoglobin-based oxygen carrier (HBOC) in human kidney normothermic perfusion. 14 discarded human kidneys were perfused for 6 hours at mean temperature of 37°C using a pressure-controlled system. Kidneys were perfused with a perfusion solution supplemented with either HBOC (n=7) or PRBC (n=7) to increase oxygen carrying capacity. Renal artery resistance, oxygen extraction, metabolic activity, energy stores, and histological features were evaluated. Throughout perfusion, kidneys from both groups exhibited comparable behavior regarding vascular flow (p=0.66), oxygen consumption (p=0.88), and reconstitution of tissue adenosine triphosphate tissue (p=0.057). Lactic acid levels were significantly higher in kidneys perfused with PRBC (p=0.007). Histological findings were comparable between groups and there was no evidence of histological damage caused by the HBOC. This feasibility experiment demonstrates that a hemoglobin-based oxygen carrier solution can offer a logistically more convenient off-the-shelf alternative to packed red blood cells in normothermic machine perfusion of human kidneys. This article is protected by copyright. All rights reserved.


Journal Details

This article was published in the following journal.

Name: American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
ISSN: 1600-6143


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