Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering.

08:00 EDT 3rd April 2019 | BioPortfolio

Summary of "Cryogenic free-form extrusion bioprinting of decellularized small intestinal submucosa for potential applications in skin tissue engineering."

In current study, a novel strategy of cryogenic 3D bioprinting assisted by free-from extrusion printing was developed and applied to printing of a decellularized small intestinal submucosa (dSIS) slurry. The rheological properties, including kinetic viscosity, storage modulus (G') and loss modulus (G''), were appropriate for free-from extrusion printing of dSIS slurry. Three different groups of scaffolds, including P<sub>500</sub>, P<sub>600</sub>, and P<sub>700</sub>, with filament distance of 500, 600, and 700 µm, respectively were fabricated at 5 mm/s working velocity of platform (Vxy) and 25 kPa air pressure of dispensing system (P) at -20ºC. The fabricated scaffolds were crosslinked via 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (EDC) which resulted in polyporous microstructure. The variations in the filament diameter and pore size were evaluated in initial frozen state after printing, lyophilized state, and after immersion in PBS solution. The Young's modulus of P<sub>500</sub>, P<sub>600</sub>, and P<sub>700</sub> scaffolds was measured in wet and dry states for EDC-crosslinked scaffolds. The cell experiment results showed improved cell adhesion, viability, and proliferation both on the surface and within the scaffold, indicating the biocompatibility and suitability of the scaffold for 3D cell models. Further, gene and protein expression of normal skin fibroblasts (NSFs) on dSIS scaffolds demonstrated their ability to promote the production of some extracellular matrix proteins (i.e. Collagen I, Collagen III, and Fibronectin)<i> in vitro</i>. Overall, this study presents a new potential strategy, by combining cryogenic 3D bioprinting with dECMs materials, to manufacture ideal scaffolds for skin tissue engineering applications.


Journal Details

This article was published in the following journal.

Name: Biofabrication
ISSN: 1758-5090


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Medical and Biotech [MESH] Definitions

A diet which is devoid of GLUTENS from WHEAT; BARLEY; RYE; and other wheat-related varieties. The diet is designed to reduce exposure to those proteins in gluten that trigger INFLAMMATION of the small intestinal mucosa in patients with CELIAC DISEASE.

A form of intestinal obstruction caused by the PROLAPSE of a part of the intestine into the adjoining intestinal lumen. There are four types: colic, involving segments of the LARGE INTESTINE; enteric, involving only the SMALL INTESTINE; ileocecal, in which the ILEOCECAL VALVE prolapses into the CECUM, drawing the ILEUM along with it; and ileocolic, in which the ileum prolapses through the ileocecal valve into the COLON.

An antennapedia-like homeodomain transcription factor that regulates the expression of multiple genes in the INTESTINAL MUCOSA. It plays a critical role in many processes from early differentiation to maintenance of the intestinal epithelial lining of both the small and large intestine.

A condition caused by the lack of intestinal PERISTALSIS or INTESTINAL MOTILITY without any mechanical obstruction. This interference of the flow of INTESTINAL CONTENTS often leads to INTESTINAL OBSTRUCTION. Ileus may be classified into postoperative, inflammatory, metabolic, neurogenic, and drug-induced.

Absorptive cells in the lining of the INTESTINAL MUCOSA. They are differentiated EPITHELIAL CELLS with apical MICROVILLI facing the intestinal lumen. Enterocytes are more abundant in the SMALL INTESTINE than in the LARGE INTESTINE. Their microvilli greatly increase the luminal surface area of the cell by 14- to 40 fold.

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