Topics

Chemoenzymatic Preparation of 4'-Thioribose NAD.

08:00 EDT 5th April 2019 | BioPortfolio

Summary of "Chemoenzymatic Preparation of 4'-Thioribose NAD."

This chemoenzymatic procedure describes a strategy for the preparation of 4'-thioribose nicotinamide adenine dinucleotide (S-NAD ), including chemical synthesis of nicotinamide 4'-riboside (S-NR), recombinant expression and purification of two NAD biosynthesis enzymes nicotinamide riboside kinase (NRK) and nicotinamide mononucleotide adenylyltransferase (NMNAT), and enzymatic synthesis of S-NAD . The first basic protocol describes the procedures for introduction of nicotinamide onto 4'-thioribose and subsequent deprotection to generate S-NR as the key intermediate for enzymatically synthesizing S-NAD . In the second basic protocol, experimental methods are detailed for the production of recombinant human NRK1 and NMNAT1 to catalyze conversion of S-NR to S-NAD . The third basic protocol presents the enzymatic approach for the generation of S-NAD from S-NR precursor. © 2019 by John Wiley & Sons, Inc.

Affiliation

Journal Details

This article was published in the following journal.

Name: Current protocols in nucleic acid chemistry
ISSN: 1934-9289
Pages: e83

Links

DeepDyve research library

PubMed Articles [2072 Associated PubMed Articles listed on BioPortfolio]

Asymmetric Chemoenzymatic Synthesis of (-)-Podophyllotoxin and Related Aryltetralin Lignans.

(-)-Podophyllotoxin is one of the most potent microtubule depolymerizing agents and has served as an important lead compound in antineoplastic drug discovery. Here, we report a short chemoenzymatic to...

Magic spot nucleotides: tunable target-specific chemoenzymatic synthesis.

A tunable chemoenzymatic strategy provides access to the entire class of magic spot nucleotides and modified analogues. The approach combines chemoselective bisphosphorylations using phosphoramidites ...

Comparison of hydroalcoholic rubbing and conventional chlorhexidine scrubbing for aseptic skin preparation in dogs.

To compare preparation time, ease of application, and elimination of skin contamination of 3 skin preparation methods for asepsis.

Skin Preparation for Prevention of Peripheral Blood Culture Contamination in Children.

In Japan, blood cultures for children are performed by pediatric residents, and povidone-iodine (PI) is the recommended solution for skin preparation. Since PI should be applied for 1.5-2 minutes befo...

Computed tomography colonography: Retrospective comparison of laxative plus barium tagging versus iodinated contrast only for bowel preparation and faecal tagging.

There is no consensus as to the most optimal bowel preparation regime for Computed Tomographic Colonography (CTC). Concerns regarding laxative bowel preparation (LBP) include tolerability, adverse eff...

Clinical Trials [894 Associated Clinical Trials listed on BioPortfolio]

Optimal Bowel Preparation Regimen in Patients With Colorectal Surgery

The study compares the efficacy of bowel cleansing between the standard preparation (2 L polyethylene glycol electrolyte solution, 2 L PEG-ELS), low-volume preparation (10 mg bisacodyl plu...

The Impact of Additional Oral Preparation on the Quality of Bowel Preparation for Colonoscopy

Although adequate bowel preparation is essential for successful colonoscopy, the 23% of patients had shown inadequate bowel preparation. Inadequate bowel preparation may results in incompl...

Improvement of Colonoscopy Preparation by Using a SPA

This study evaluates the impact of a SPA on Quality of bowel preparation for colonoscopy. Half of the patients receive regular paper based information on colonoscopy preparation (control),...

Personalized Colonoscopy Preparation for Patients With Risk Factors for Inadequate Preparation: A Randomized Control Trial

Adequate bowel preparation is one of the most important quality measures in colonoscopy. Previous studies identified several factors associated with inadequate bowel preparation (IBP). No ...

A Randomized Controlled Trial of Tailored Bowel Preparation Based on A Predictive Model

We aim to develop a predictive model to distinguish patients at risk for inadequate bowel preparation, and to compare adequate bowel preparation rate, adenoma detection rate and adverse ev...

Medical and Biotech [MESH] Definitions

A pharmaceutical preparation containing a mixture of water-soluble, conjugated estrogens derived wholly or in part from URINE of pregnant mares or synthetically from ESTRONE and EQUILIN. It contains a sodium-salt mixture of estrone sulfate (52-62%) and equilin sulfate (22-30%) with a total of the two between 80-88%. Other concomitant conjugates include 17-alpha-dihydroequilin, 17-alpha-estradiol, and 17-beta-dihydroequilin. The potency of the preparation is expressed in terms of an equivalent quantity of sodium estrone sulfate.

Facilities for the preparation and dispensing of drugs.

A preparation of hog pancreatic enzymes standardized for lipase content.

Collections of recipes or instructions for preparation of food and organization of meals.

The art or practice of cooking. It includes the preparation of special foods for diets in various diseases.

Quick Search


DeepDyve research library

Relevant Topic

Recombinant DNA
Recombinant DNA is the formation of a novel DNA sequence by the formation of two DNA strands. These are taken from two different organisms. These recombinant DNA molecules can be made with recombinant DNA technology. The procedure is to cut the DNA of ...


Searches Linking to this Article