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The spatial dimensions of host cells and bacterial microbes are perfectly suited to being studied by microscopy techniques. Therefore, cellular imaging has been instrumental in uncovering many paradigms of the intracellular lifestyle of microbes. Initially, microscopy was used as a qualitative, descriptive tool. However, with the onset of specific markers and the power of computer-assisted image analysis, imaging can now be used to gather quantitative data on biological processes. This makes imaging a driving force for the study of cellular phenomena. One particular imaging modality stands out, which is based on the physical principles of fluorescence. Fluorescence is highly specific and therefore can be exploited to label biomolecules of choice. It is also very sensitive, making it possible to follow individual molecules with this approach. Also, microscopy hardware has played an important role in putting microscopy in the spotlight for host-pathogen investigations. For example, microscopes have been automated for microscopy-based screenings. A new generation of microscopes and molecular probes are being used to image events below the resolution limit of light. Finally, workflows are being developed to link light microscopy with electron microscopy methods via correlative light electron microscopy. We are witnessing a golden age of cellular imaging in cellular microbiology.
This article was published in the following journal.
Name: Microbiology spectrum
Regulated cell death via apoptosis not only is important for organismal homeostasis but also serves as an innate defense mechanism. The engulfment of apoptotic infected cells, a process known as effer...
Hypoxia-inducible transcription factor-1α (HIF-1α) was originally identified as a master regulator of cellular responses to hypoxia. More recently, HIF-1α has emerged as a critical regulator of imm...
Transition metal ions are essential to bacterial pathogens and their hosts alike but are harmful in excess. In an effort to curtail the replication of intracellular bacteria, host phagocytes exploit b...
Inflammasomes are a formidable armada of intracellular pattern recognition receptors. They recognize determinants of infection, such as foreign entities or danger signals within the host cell cytosol,...
Yersinia pestis is able to survive and replicate within macrophages, while also being able to live in the extracellular milieu of the host. Assays that facilitate better understanding of how Y. pestis...
Observational: studies in human beings in which biomedical and/or health outcomes are assessed in pre-defined groups of individuals. Subjects in the study may receive diagnostic, therapeut...
To compare all-cause mortality at Day 14 of S-649266 with that of the comparator, meropenem, in adults with hospital-acquired bacterial pneumonia (HABP), ventilator-associated bacterial pn...
This project aimed at analyzing and assessing of the early and late Hospital-Acquired Pneumonia(HAP) bacterial pathogens and their resistance to antimicrobial agents. The incidence of thei...
Many studies provide evidence for the benefit of lower respiratory tract surveillance, mostly by culture of endotracheal secretions, to predict bacterial pathogens (especially multi-drugs ...
This study will evaluate the effect of skin antisepsis and/or emollient therapy on bacterial colonization dynamics in very low birth weight, hospitalized infants. Bacterial swabs from 5 bo...
The use of molecularly targeted imaging probes to localize and/or monitor biochemical and cellular processes via various imaging modalities that include RADIONUCLIDE IMAGING; ULTRASONOGRAPHY; MAGNETIC RESONANCE IMAGING; fluorescence imaging; and MICROSCOPY.
A pattern recognition receptor that binds unmethylated CPG CLUSTERS. It mediates cellular responses to bacterial pathogens by distinguishing between self and bacterial DNA.
A pattern recognition receptor that binds FLAGELLIN. It mediates cellular responses to certain bacterial pathogens.
A mitogen-activated protein kinase subfamily that regulates a variety of cellular processes including CELL GROWTH PROCESSES; CELL DIFFERENTIATION; APOPTOSIS; and cellular responses to INFLAMMATION. The P38 MAP kinases are regulated by CYTOKINE RECEPTORS and can be activated in response to bacterial pathogens.
A large family of cell surface receptors that bind conserved molecular structures present in pathogens. They play important roles in host defense by mediating cellular responses to pathogens.
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...
Microbiology (from Greek μῑκρος, mīkros, "small"; βίος, bios, "life"; and -λογία, -logia) is the study of microscopic organisms, either unicellular (singl...