Track topics on Twitter Track topics that are important to you
In traditional Chinese medicine, Lysimachiae Herba (LH) and Desmodii Styracifolii Herba (DSH) have been widely used for the treatment of calculi, but there is a certain focus in clinical application. Flavonoids as their pharmacologically active substances were focusly studied to make clear of their chemical compositions and reveal the similarities and differences between LH and DHS by analysis of characteristic marker components at the molecular level. An ultra high performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry (UPLC-QTOF-MS/MS) approach based on metabolite profiling was established. The high-resolution data was acquired through data dependent acquisition (DDA) mode. Based on the targeted and untargeted analytical strategies, a total of 113 compounds were identified, of which 80 compounds existed in LH and 61 in DSH. Then multivariate statistical analysis was applied to further find the characteristic marker components, and a total number of 21 variables were screened as the valuable variables for discrimination. By matching with identified flavonoids, these 21 variables were corresponding to 15 flavonoids (including 6 from LH and 9 from DSH) which were firstly identified as the marker compounds. These results indicated that the UPLC-QTOF-MS/MS method with analysis strategy was a powerful tool for rapidly identification and screening of marker compounds of flavonoids between LH and DSH, and the 15 screened marker compounds provide a chemical basis for the further researches on the mechanisms of LH and DSH in the treatment of cholelithiasis and nephrolithiasis respectively.
This article was published in the following journal.
Name: Journal of pharmaceutical and biomedical analysis
Dietary flavonoids are known as scavengers of reactive oxygen species such as hypochlorous acid. In spite of abundant scavenging capacity data reported, few reports have addressed the relationship bet...
Polysaccharides are a major class of biomacromolecules. Their bioactivities depend on chemical structure, which includes monosaccharide composition, linkages below sugar residues, and solution conform...
The actin cytoskeleton is dysregulated in cancer, yet this critical cellular machinery has not translated as a druggable clinical target due to cardio-toxic side-effects. Many actin regulators are als...
Flavonoids are natural polyphenolic compounds which are included in a panoply of drugs and used to treat and/or manage human diseases such as metabolic, cardiovascular, neurological diseases and cance...
The response of plants to grazing includes the production of chemical defense compounds such as proteases inhibitors and secondary metabolites as flavonoids, which makes them less palatable to feeding...
Aim of the study is to investigate the effect of a 12-week supplementation of Santa Herba Extract on body weight in overweight and obese subjects. Additionally appetite related marker as w...
To determine the association between flavonoids intake and the prevention of incident cardiovascular disease (CVD).
The goal of this screening portion of this clinical research study is to learn if you are eligible to take part in a clinical research study using Tarceva (erlotinib hydrochloride) and eit...
The study aimed to assess the safety and, partially, the efficacy of dietary supplementation of a flavonoids-, DHA- and EPA-based natural supplement in non-ambulant DMD boys and in a cohor...
The study use a double blind, placebo-controlled design enrolling male and female subjects between 55-90 yo to evaluate the effect of daily consumption of a cocoa beverage on anthropometri...
Examination of urine by chemical, physical, or microscopic means. Routine urinalysis usually includes performing chemical screening tests, determining specific gravity, observing any unusual color or odor, screening for bacteriuria, and examining the sediment microscopically.
The identification of selected parameters in newborn infants by various tests, examinations, or other procedures. Screening may be performed by clinical or laboratory measures. A screening test is designed to sort out healthy neonates (INFANT, NEWBORN) from those not well, but the screening test is not intended as a diagnostic device, rather instead as epidemiologic.
A mixture of flavonoids extracted from seeds of the MILK THISTLE, Silybum marianum. It consists primarily of silybin and its isomers, silicristin and silidianin. Silymarin displays antioxidant and membrane stabilizing activity. It protects various tissues and organs against chemical injury, and shows potential as an antihepatoxic agent.
Testing or screening required by federal, state, or local law or other agencies for the diagnosis of specified conditions. It is usually limited to specific populations such as categories of health care providers, members of the military, and prisoners or to specific situations such as premarital examinations or donor screening.
Rapid methods of measuring the effects of an agent in a biological or chemical assay. The assay usually involves some form of automation or a way to conduct multiple assays at the same time using sample arrays.
Complementary and Alternative Medicine
Alternative medicine are whole medical systems that did not fit with conventional medicine as they have completely different philosophies and ideas on the causes of disease, methods of diagnosis and approaches to treatment. Although often overlapping, co...
Multiple Sclerosis MS
Multiple sclerosis (MS) is the most common disabling neurological condition affecting 100,000 young adults in the UK. The condition results from autoimmune damage to myelin, causing interference in nerve signaling. Symptoms experienced depend on the pa...