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Real-time pure shift measurements for uniformly isotope-labeled molecules using X-selective BIRD homonuclear decoupling.

08:00 EDT 30th March 2019 | BioPortfolio

Summary of "Real-time pure shift measurements for uniformly isotope-labeled molecules using X-selective BIRD homonuclear decoupling."

We introduce a novel selective inversion element for chunked homonuclear decoupling that combines isotope selection via BIRD-filtering with band-selective inversion on the X-heteronucleus and allows efficient real-time decoupling of homonuclear and heteronuclear couplings. It is especially suitable for uniformly isotope-labeled compounds. We discuss in detail the inversion element based on band-selective refocusing on the X-nuclei (BASEREX), highlighting in particular the role of appropriate band-selective shaped refocusing pulses and the application of broadband X-pulses for an effective BIRD element during homodecoupling. The approach is experimentally verified and studied in detail using uniformly C-labeled glucose and a uniformly N,C-labeled amino acid mixture.

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This article was published in the following journal.

Name: Journal of magnetic resonance (San Diego, Calif. : 1997)
ISSN: 1096-0856
Pages: 64-71

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