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The accumulation of chemical compounds in fish tissue represents significant health concerns for seafood consumers, but little is known about the risks to human health associated with such substances. The identification of adverse biological responses upon exposure to contaminants has been facilitated by the development of in vitro systems resembling the human dietary pathway. The present study explores the applicability of an organotypic co-culture system, using intestinal (Caco-2) and hepatic (HepaRG) cell lines, to provide insight into the toxicity of chemical mixtures found in commercially available seafood. Chemical extractions were conducted utilizing fish and oyster standard reference material (SRM) from the U.S. National Institute of Standards and Technology (NIST). Cells were seeded in monoculture and co-culture systems and exposed to SRM extracts before measurements of cytotoxicity and antioxidant responses. Exposure to oyster extracts led to significant cell mortality in monocultures. HepaRG cells in monoculture expressed lower levels of glutathione peroxidase and superoxide dismutase than HepaRG cells in co-culture, upon exposure to both oyster and fish extracts. These observations illustrate the importance of organotypic co-culture models to explore biological responses that could be otherwise difficult to evaluate in monocultures, and the adverse effects associated with the consumption of contaminated seafood.
This article was published in the following journal.
Many cell culture and animal models have been used to study hepatitis B virus (HBV) replication and its effects in the liver; these have facilitated development of strategies to control and clear chro...
Multicellular organisms balance oxygen delivery and toxicity by having oxygen pass through several barriers before cellular delivery. In human cell culture, these physiologic barriers are removed, exp...
To develop a culture regime for the in vitro human lens capsular bag model that better reflects clinical events following cataract surgery and to use this refined model to evaluate the putative impact...
/Objective: The conventional models currently used to evaluate various anti-tumor therapeutic agents are not sufficient for representing human pancreatic ductal adenocarcinoma (PDA), which has a uniqu...
Malathion is one of the most widely used organophosphorus insecticides in agriculture. However, malathion may be involved in the etiology of human brain dysfunction. Induction of ROS has been proposed...
The first aim of the study is to obtain from chirurgical micro biopsy myoblast in culture which will differentiate in Diaphragm fiber. Then these fibers will be submitted under mechanical ...
Recent developments in the understanding of stem- and progenitor cell differentiation raises hopes that brain damage in chronic neurological diseases may become repaired by systemic or foc...
Myriads of elements affect human embryo culture in vitro. These include, but not limited to, air quality, culture media, culture temperature, incubator humidity, pH, and O2 levels. O2 tens...
Plasmodium falciparum isolates display a wide genetic diversity with possibly different properties to induce immune responses. These properties could directly influence the ability to indu...
This current three-year proposal aims to clarify the mechanisms of melanocyte destruction and regeneration in vitiligo lesions using both traditional cell culture and skin equivalent model...
Statistical formulations or analyses which, when applied to data and found to fit the data, are then used to verify the assumptions and parameters used in the analysis. Examples of statistical models are the linear model, binomial model, polynomial model, two-parameter model, etc.
A species of gram-negative bacteria that is a common inhabitant of the human OROPHARYNX and also cell culture contaminant. It is usually non-pathogenic but it can cause infections in leukemic or immunosuppressed patients.
Spherical, heterogeneous aggregates of proliferating, quiescent, and necrotic cells in culture that retain three-dimensional architecture and tissue-specific functions. The ability to form spheroids is a characteristic trait of CULTURED TUMOR CELLS derived from solid TUMORS. Cells from normal tissues can also form spheroids. They represent an in-vitro model for studies of the biology of both normal and malignant cells. (From Bjerkvig, Spheroid Culture in Cancer Research, 1992, p4)
CULTURE MEDIA free of serum proteins but including the minimal essential substances required for cell growth. This type of medium avoids the presence of extraneous substances that may affect cell proliferation or unwanted activation of cells.
A technique for maintaining or growing TISSUE in vitro, usually by DIFFUSION, perifusion, or PERFUSION. The tissue is cultured directly after removal from the host without being dispersed for cell culture.
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...