The effects of dietary Bacillus cereus QSI-1 on skin mucus proteins profile and immune response in Crucian Carp (Carassius auratus gibelio).

08:00 EDT 7th April 2019 | BioPortfolio

Summary of "The effects of dietary Bacillus cereus QSI-1 on skin mucus proteins profile and immune response in Crucian Carp (Carassius auratus gibelio)."

The objective of this study was to investigate the effect of dietary quorum quenching bacterium Bacillus cereus QSI-1 on skin mucus protein pattern and innate immune response in Crucian Carp (Carassius auratus gibelio). The differential proteomes of skin mucus of Crucian Carp were analyzed after administration of Bacillus cereus QSI-1 by isobaric tags for relative and absolute quantitation (iTRAQ) labeling, followed by liquid chromatography-tandem mass spectrometry (LC-MS/MS). A total of 1974 proteins were quantified. Using a 1.5-fold change in expression as a physiological significant benchmark, 264 differentially expressed proteins were reliably quantified by iTRAQ analysis, including 130 up- and 134 down-regulated proteins after dietary Bacillus cereus QSI-1. Some Proteins that were involved in immunity included protein S100, annexin, histone H3, lymphocyte cytosolic protein 1, heat shock protein, L-plastin, keratin 91, etc. Furthermore, fish fed 5 × 10 CFU/g Bacillus cereus QSI-1 supplemented diet showed an increase in alternative complement activity and lysozyme activity but expressed a decrease in superoxide dismutase activity in skin mucus (P < 0.05). However, administration of Bacillus cereus QSI-1 had no significant effects on total immunoglobulin level (P > 0.05). These results demonstrated that dietary administration of Bacillus cereus QSI-1 affects skin mucus protein profile and innate immune response in Crucian Carp, and also can enhance the disease resistance of Crucian Carp against A. hydrophila. This is the first report on proteomics analysis of skin mucus proteins in Crucian Carp after administration of quorum quenching bacterium Bacillus cereus, and the results will help to understand the mucosal immune responses to probiotics at the protein level in fish.


Journal Details

This article was published in the following journal.

Name: Fish & shellfish immunology
ISSN: 1095-9947


DeepDyve research library

PubMed Articles [25117 Associated PubMed Articles listed on BioPortfolio]

Whole genome sequence of Bacillus thuringiensis ATCC 10792 and improved discrimination of Bacillus thuringiensis from Bacillus cereus group based on novel biomarkers.

Among the Bacillus cereus group, B. thuringiensis, is one of the most extensively used biological control agent. The present study reports the complete genome and four novel plasmid analysis of the ty...

Effect of alternating current and Bacillus cereus on the stress corrosion behavior and mechanism of X80 steel in a Beijing soil solution.

The effects of alternating current (AC) and Bacillus cereus (B. cereus) on the stress corrosion behavior and mechanism of X80 pipeline steel were investigated in a Beijing soil solution in this study....

Co-infection of Clostridioides (Clostridium) difficile GMU1 and Bacillus cereus GMU2 in one patient in Guizhou, China.

Clostridioides (Clostridium) difficile and Bacillus cereus infections are frequently reported in human individually. However, co-infection of both pathogens in human is extremely rare. In the present ...

Analysis of a newly discovered antigen of Bacillus cereus biovar anthracis for its suitability in specific serological antibody testing.

The aim of this work was to identify a protein which can be used for specific detection of antibodies against Bacillus cereus biovar anthracis (Bcbva), an anthrax-causing pathogen that so far has been...

Biology, environmental and nutritional modulation of skin mucus alkaline phosphatase in fish: A review.

Alkaline phosphatase (AP) is a major, recently recognized component of innate immunity. The intestinal AP (IAP) isoform plays a pivotal role in controlling gastrointestinal and systemic inflammation i...

Clinical Trials [9884 Associated Clinical Trials listed on BioPortfolio]

A Study Evaluating Bacillus Cereus in the Prevention of Afatinib-associated Diarrhea

Afatinib is an irreversible ErbB-family blocker with approved clinical activity in non-small-cell lung cancer (NSCLC) with EGFR mutations. It has received regulatory approval for use as a ...

Description of the Pathogenicity and Incriminated Modes of Contamination of Neonatal Bacillus Species Sepsis.

Bacillus species are ubiquitous gram-positive spore-forming organisms. They rarely cause disease in the immunocompetent and are more frequently isolated as a culture contaminant. However, ...

Impact of Bacillus Clausii Administration on Irritable Bowel Syndrome Patients

Probiotics have been proposed as useful therapeutic alternatives for the treatment of irritable bowel syndrome patients. Bacillus clausii is a probiotic that has been used for the treatmen...

Clinical Effects of Bacillus Containing Oral Hygiene Products on Gingivitis: a Randomized Controlled Trial

Aim: Evaluating the clinical effects of a Bacillus subtilis, Bacillus megaterium and Bacillus pumulus containing toothpaste, mouthwash and toothbrush cleaner versus a placebo in patients w...

Acute Effects of Dietary Proteins on Postprandial Lipemia, Incretin Responses and Subclinical Inflammation in Obese Subjects

The purpose of this study is to determine the effects of dietary protein on blood lipids and gut hormones after a fat-rich meal. Hypothesis: Certain dietary proteins reduce the amount of ...

Medical and Biotech [MESH] Definitions

A species of rod-shaped bacteria that is a common soil saprophyte. Its spores are widespread and multiplication has been observed chiefly in foods. Contamination may lead to food poisoning.

A metalloproteinase which degrades helical regions of native collagen to small fragments. Preferred cleavage is -Gly in the sequence -Pro-Xaa-Gly-Pro-. Six forms (or 2 classes) have been isolated from Clostridium histolyticum that are immunologically cross-reactive but possess different sequences and different specificities. Other variants have been isolated from Bacillus cereus, Empedobacter collagenolyticum, Pseudomonas marinoglutinosa, and species of Vibrio and Streptomyces. EC

Iron or iron compounds used in foods or as food. Dietary iron is important in oxygen transport and the synthesis of the iron-porphyrin proteins hemoglobin, myoglobin, cytochromes, and cytochrome oxidase. Insufficient amounts of dietary iron can lead to iron-deficiency anemia.

Method to determine the occurrence of OVULATION by direct or indirect means. Indirect methods examine the effects of PROGESTERONE on cervical mucus (CERVIX MUCUS), or basal body temperature. Direct ovulation detection, generally used in fertility treatment, involves analyses of circulating hormones in blood and ULTRASONOGRAPHY.

Viruses whose host is Bacillus. Frequently encountered Bacillus phages include bacteriophage phi 29 and bacteriophage phi 105.

Quick Search


DeepDyve research library

Relevant Topics

Wound management
Anything that breaks the skin is a wound because when the skin is broken, there's a risk of germs getting into the body and causing an infection. Follow and track Wound Care News on BioPortfolio: Wound Car...

Acne Dermatology Eczema Psoriasis Wound Care Dermatology is the medical specialty concerned with the diagnosis and treatment of skin disorders (Oxford Medical Dictionary). As well as studying how the skin works, dermatology covers...

Searches Linking to this Article