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Autonomous patch clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex.

08:00 EDT 10th April 2019 | BioPortfolio

Summary of "Autonomous patch clamp robot for functional characterization of neurons in vivo: development and application to mouse visual cortex."

Patch clamping is the gold standard measurement technique for cell type characterization in vivo but it is low throughput, difficult to scale, and requires highly skilled operation. We developed an autonomous robot that can acquire multiple consecutive patch clamp recordings in vivo. In practice, 40 pipettes loaded into a carousel are sequentially filled and inserted into the brain, localized to a cell, used for patch clamping, and disposed. Automated visual stimulation and electrophysiology software enables functional cell type classification of whole cell patched cells, as we show for 37 cells in the anesthetized mouse in visual cortex (V1) L5. We achieved 9% yield, with 5.3 min per attempt over hundreds of trials. The highly variable and low yield nature of in vivo patch clamp recordings will benefit from such a standardized, automated, quantitative approach, allowing development of optimal algorithms and enabling scaling required for large scale studies and integration with complementary techniques.

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This article was published in the following journal.

Name: Journal of neurophysiology
ISSN: 1522-1598
Pages:

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Medical and Biotech [MESH] Definitions

An electrophysiologic technique for studying cells, cell membranes, and occasionally isolated organelles. All patch-clamp methods rely on a very high-resistance seal between a micropipette and a membrane; the seal is usually attained by gentle suction. The four most common variants include on-cell patch, inside-out patch, outside-out patch, and whole-cell clamp. Patch-clamp methods are commonly used to voltage clamp, that is control the voltage across the membrane and measure current flow, but current-clamp methods, in which the current is controlled and the voltage is measured, are also used.

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Nerve structures through which impulses are conducted from a nerve center toward a peripheral site. Such impulses are conducted via efferent neurons (NEURONS, EFFERENT), such as MOTOR NEURONS, autonomic neurons, and hypophyseal neurons.

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