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Gaining confidence in the elusive histidine phosphoproteome.

08:00 EDT 10th April 2019 | BioPortfolio

Summary of "Gaining confidence in the elusive histidine phosphoproteome."

Recent technological advances have made it possible to investigate the hitherto rather elusive protein histidine phosphorylation. However, confident site-specific localization of protein histidine phosphorylation remains challenging. Here, we address this problem presenting a mass spectrometry-based approach outperforming classical HCD fragmentation without compromising sensitivity. We use the phosphohistidine immonium ion as a diagnostic tool as well as ETD-based fragmentation techniques to achieve unambiguous identification and localization of histidine phosphorylation sites. The work presented here will allow the more confident investigation of the phosphohistidine proteome to reveal the roles of histidine phosphory-lation in cellular signaling events.

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This article was published in the following journal.

Name: Analytical chemistry
ISSN: 1520-6882
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Medical and Biotech [MESH] Definitions

An enzyme that catalyzes the first step of histidine catabolism, forming UROCANIC ACID and AMMONIA from HISTIDINE. Deficiency of this enzyme is associated with elevated levels of serum histidine and is called histidinemia (AMINO ACID METABOLISM, INBORN ERRORS).

A member of the transferase superfamily of proteins. In the activated state, protein-histidine kinase autophosphorylates at a histidine residue, subsequently transferring high-energy phosphoryl groups to an aspartate residue of the response-regulator domain, which results in a conformational shift in the effector domain. Histidine kinases mediate signal transduction in a wide range of processes involving cellular adaptation to environmental stress.

An enzyme that activates histidine with its specific transfer RNA. EC 6.1.1.21.

An enzyme that catalyzes the decarboxylation of histidine to histamine and carbon dioxide. It requires pyridoxal phosphate in animal tissues, but not in microorganisms. EC 4.1.1.22.

The penultimate step in the pathway of histidine biosynthesis. Oxidation of the alcohol group on the side chain gives the acid group forming histidine. Histidinol has also been used as an inhibitor of protein synthesis.

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