Advertisement

Topics

The structure of the elusive urease-urea complex unveils a paradigmatic case of metallo-enzyme catalysis.

08:00 EDT 10th April 2019 | BioPortfolio

Summary of "The structure of the elusive urease-urea complex unveils a paradigmatic case of metallo-enzyme catalysis."

Urease, the most efficient enzyme known, contains an essential dinuclear Ni(II) cluster in the active site. It catalyses the hydrolysis of urea, inducing a rapid pH increase that has negative effects on human health and agriculture. Thus, control of urease activity is of utmost importance in medical, pharmaceutical, and agro-environmental applications. All known urease inhibitors are either toxic or inefficient. The development of new and efficient chemicals able to inhibit urease relies on the knowledge of all steps of the catalytic mechanism. The short (microseconds) lifetime of the urease-urea complex has hampered the obtainment of its structure. This study uses fluoride to substitute the hydroxide acting as the co-substrate in the reaction, preventing the occurrence of the catalytic steps that follow substrate binding. The 1.42-Å crystal structure of the urease-urea complex, here reported, resolves the enduring debate on the mechanism of this paradigmatic metallo-enzyme.

Affiliation

Journal Details

This article was published in the following journal.

Name: Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Pages:

Links

DeepDyve research library

PubMed Articles [15561 Associated PubMed Articles listed on BioPortfolio]

Progress in bacterial urease complexes and their activation mechanisms.

Urease decomposes urea to ammonia, and has application potential in agriculture and medical treatment. Urease proteins include structural proteins (UreA, UreB and UreC) and accessory proteins (UreD/Ur...

Long-term urea fertilization alters the composition and increases the abundance of soil ureolytic bacterial communities in an upland soil.

Urea is a widely used nitrogen fertilizer in agriculture, but considerable amounts of urea are lost through ammonia volatilization. Soil microbes are major urease producers; however, the impact of ure...

Urease is an essential component of the acid response network of Staphylococcus aureus and is required for a persistent murine kidney infection.

Staphylococcus aureus causes acute and chronic infections resulting in significant morbidity. Urease, an enzyme that generates NH3 and CO2 from urea, is key to pH homeostasis in bacterial pathogens un...

Syntheses, in vitro urease inhibitory activities of urea and thiourea derivatives of tryptamine, their molecular docking and cytotoxic studies.

Urease is an enzyme of amidohydrolase family and is responsible for the different pathological conditions in the human body including peptic ulcers, catheter encrustation, kidney stone formation, hepa...

Food-grade expression of an iron-containing acid urease in Bacillus subtilis.

Enzymatic degradation of urea, the precursor of carcinogenic compound ethylcarbamate in rice wine, is always attractive. In the present study, we achieved high efficient production of Bacillus paralic...

Clinical Trials [2975 Associated Clinical Trials listed on BioPortfolio]

Urease Inhibitor Drug Treatment for Urea Cycle Disorders

The purpose of this study is to determine if acetohydroxamic acid (AHA) can prevent hydrolysis of urea by inhibiting the bacterial urease of gut flora of both healthy control adults as wel...

Rapid Urease Test for Diagnosis Helicobacter Pylori Infection

Nowadays, the gold standard examinations for diagnosing H. pylori infection are histopathology and culture examination. However, those examinations take long preparation so they are not su...

Study to Evaluate 13 C Isotope Ratio Measurement for Urea Cycle Capacity Assessment

In this short-term study a method for the evaluation of the metabolic competency of the urea cycle in vivo will be assessed. In order to monitor the efficacy of new treatment options for p...

Study of Treatment and Metabolism in Patients With Urea Cycle Disorders

RATIONALE: The urea cycle is the process in which nitrogen is removed from the blood and converted into urea, a waste product found in urine . Urea cycle disorders are inherited disorders ...

Performance Evaluation of In-Vitro Diagnostic Device

This is an interim report for POC testing of skyla Clinical Chemistry Analyzer and four skyla Test Systems (ALB, BUN, GLU, and TP). The analyses are performed with skyla Clinical Chemistry...

Medical and Biotech [MESH] Definitions

An enzyme that catalyzes the conversion of urea and water to carbon dioxide and ammonia. EC 3.5.1.5.

The urea concentration of the blood stated in terms of nitrogen content. Serum (plasma) urea nitrogen is approximately 12% higher than blood urea nitrogen concentration because of the greater protein content of red blood cells. Increases in blood or serum urea nitrogen are referred to as azotemia and may have prerenal, renal, or postrenal causes. (From Saunders Dictionary & Encyclopedia of Laboratory Medicine and Technology, 1984)

An amino acid produced in the urea cycle by the splitting off of urea from arginine.

The genetic region which contains the loci of genes which determine the structure of the serologically defined (SD) and lymphocyte-defined (LD) TRANSPLANTATION ANTIGENS, genes which control the structure of the IMMUNE RESPONSE-ASSOCIATED ANTIGENS, HUMAN; the IMMUNE RESPONSE GENES which control the ability of an animal to respond immunologically to antigenic stimuli, and genes which determine the structure and/or level of the first four components of complement.

A complex of proteins that assemble the SNRNP CORE PROTEINS into a core structure that surrounds a highly conserved RNA sequence found in SMALL NUCLEAR RNA. They are found localized in the GEMINI OF COILED BODIES and in the CYTOPLASM. The SMN complex is named after the Survival of Motor Neuron Complex Protein 1, which is a critical component of the complex.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Enzymes
Enzymes are proteins that catalyze (i.e., increase the rates of) chemical reactions. In enzymatic reactions, the molecules at the beginning of the process, called substrates, are converted into different molecules, called products. Almost all chemical re...


Searches Linking to this Article