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Electrochemical Monitoring of ROS/RNS Homeostasis Within Individual Phagolysosomes Inside Single Macrophages.

08:00 EDT 10th April 2019 | BioPortfolio

Summary of "Electrochemical Monitoring of ROS/RNS Homeostasis Within Individual Phagolysosomes Inside Single Macrophages."

Reactive Oxygen/Nitrogen Species (ROS/RNS) produced by macrophages inside their phagolysosomes are closely related to immunity and inflammation by being involved in the removal of pathogens, altered cells, etc. The existence of a homeostatic mechanism regulating the ROS/RNS amounts inside phagolysosomes has been invoked to account for the efficiency of this crucial process but this could never be unambiguously documented. In this work, intracellular electrochemical analysis with platinized nanowires electrodes (Pt-NWEs) allowed monitoring ROS/RNS effluxes with sub-millisecond resolution from individual phagolysosomes randomly impacting onto the electrode inserted inside a living macrophage. This evidenced for the first time that the consumption of ROS/RNS by their oxidation at the nanoelectrode surface stimulates the production of significant ROS/RNS amounts inside phagolysosomes. These results established the existence of the long-time postulated ROS/RNS homeostasis and allowed quantifying its kinetics and efficiency. ROS/RNS concentrations may then be maintained at sufficiently high levels for sustaining proper pathogen digestion rates without endangering the macrophage internal structures.

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This article was published in the following journal.

Name: Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
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