Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy.

07:00 EST 1st January 2019 | BioPortfolio

Summary of "Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy."

Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane.


Journal Details

This article was published in the following journal.

Name: Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Pages: 337-348


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