Advertisement

Topics

Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy.

07:00 EST 1st January 2019 | BioPortfolio

Summary of "Analysis of Spatial Assembly of GPCRs Using Photoactivatable Dyes and Localization Microscopy."

Super-resolution imaging has provided unprecedented insight in the molecular complexities of fundamental cell biological questions. For G protein-coupled receptors (GPCRs), its application to the study of receptor homomers and heteromers have unveiled the diversity of complexes these GPCRs can form at the plasma membrane at a structural and functional level. Here, we describe our methodological approach of photoactivated localization microscopy with photoactivatable dyes (PD-PALM) to visualize and quantify the spatial assembly of GPCR heteromers at the plasma membrane.

Affiliation

Journal Details

This article was published in the following journal.

Name: Methods in molecular biology (Clifton, N.J.)
ISSN: 1940-6029
Pages: 337-348

Links

DeepDyve research library

PubMed Articles [18644 Associated PubMed Articles listed on BioPortfolio]

A Photoactivatable BODIPY Probe for Localization-Based Super-Resolution Cellular Imaging.

The synthesis and application of a photoactivatable boron-alkylated BODIPY probe for localization-based super-resolution microscopy is reported. Photoactivation and excitation of the probe is achieved...

Far-Red Photoactivatable BODIPYs for the Super-Resolution Imaging of Live Cells.

The photoinduced disconnection of an oxazine heterocycle from a borondipyrromethene (BODIPY) chromophore, equipped with a styryl appendage, activates bright far-red fluorescence. The high brightness o...

Super-Resolution Imaging of G Protein-Coupled Receptors Using Ground State Depletion Microscopy.

G protein-coupled receptors (GPCRs) comprise the largest family of integral membrane proteins, which are coupled to heterotrimeric G proteins to influence cell signaling. Subsequent to G protein activ...

Spatial phylogenetics reveals evolutionary constraints on the assembly of a large regional flora.

We used spatial phylogenetics to analyze the assembly of the Wisconsin flora, linking processes of dispersal and niche evolution to spatial patterns of floristic and phylogenetic diversity and testing...

Monitoring the Aggregation of GPCRs by Fluorescence Microscopy.

G protein-coupled receptors (GPCRs) contain highly hydrophobic domains that are subject to aggregation when exposed to the crowded environment of the cytoplasm. Many events can lead to protein aggrega...

Clinical Trials [2390 Associated Clinical Trials listed on BioPortfolio]

Spatial Hearing Perception in Bilateral Cochlear Implant Children

Spatial hearing in cochlear implant (CI) users is a challenging investigation field since no studies have explored yet spatial auditory perception in three-dimensional space (3D). Moreover...

The Mechanism of Melanocyte Self-Assembly on Biomaterials and the Functional Analysis

The aim of this study is to survey melanocytes in the ability of self-assembly on biomaterials and functional analysis in vitro. Also, this study may provide a better way for treatment of ...

Clinical Investigation to Evaluate the Haemonetics POLFA Modified Sample Needle Assembly With Vacuum Tube Holder

This study evaluates whether whole blood transferred through the new POLFA needle assembly meets supernatant hemoglobin acceptability standards.

Electrohaemodynamic Activity in Epileptic Children : Combined Video-EEG and Near-infrared Spectroscopy (NIRS) Analysis

This study is designed to evaluate the contribution of multimodal high-resolution EEG-NIRS electrical and local haemodynamic analysis (HR-EEG, HR-NIRS) to spatial localization of epileptog...

Localization and Mismatch Negativity

This descriptive and observational research project aims to characterize MMN as a neuronal marker of localization deficit in single-sided deafened subjects and subjects with bilateral prof...

Medical and Biotech [MESH] Definitions

Methods used to take into account and incorporate spatial autocorrelation and regional variation into regression analysis models of data that has spatial dependency, and also to provide information on the spatial relationships among the variables.

Non-visual system arrestins that negatively regulate G-PROTEIN-COUPLED RECEPTORS (GPCRs) and may also function independently of GPCR signaling. They bind and recruit many different signaling factors, including MITOGEN-ACTIVATED PROTEIN KINASES; SRC-FAMILY-KINASES; and FILAMIN to GPCRs and may recognize different phosphorylation states of the receptors to determine the specificity of the cellular response to signaling.

Investigative techniques which measure the topological, geometric, and or geographic properties of the entities studied.

Integration of spatial information perceived by visual and/or auditory CUES.

A process through which individuals encode information about their environmental CUES to facilitate SPATIAL NAVIGATION.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Biological Therapy
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...

Nutrition
Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...


Searches Linking to this Article