Fabrication of gold-silver core-shell nanoparticles for performing as ultrabright SERS-nanotags inside human ovarian cancer cells.

08:00 EDT 11th April 2019 | BioPortfolio

Summary of "Fabrication of gold-silver core-shell nanoparticles for performing as ultrabright SERS-nanotags inside human ovarian cancer cells."

This paper presents the fabrication and characterization of new gold-silver core-shell nanoparticles labeled with para-mercaptobenzoic acid (4MBA) molecules and demonstrates their use as SERS-nanotags with ultra-bright traceability inside cells and ability to convey spectrally-coded information about the intracellular pH by means of Surface-Enhanced Raman Spectroscopy (SERS). Unlike to previous reported studies, our fabrication procedure includes in the first step the synthesis of chitosan-coated gold nanoparticles as a seed material with subsequent growing of a silver shell. The bimetallic core-shell structure is revealed by transmission electron microscopy (TEM), high-angle annular dark field scanning transmission electron microscopy (HAADF-STEM), energy-dispersive X-ray elemental mapping (EDX) and the presence of two interacting localized surface plasmon resonance (LSPR) modes in UV-Vis extinction spectrum. The high SERS activity and sensitivity of as fabricated 4MBA-chit-Au-AgNPs nano-constructs to different pH in solution is investigated under 532 and 633 nm laser lines excitation. Next, in view of future studies in cancer diagnosis, the in vitro antiproliferative effects of SERS-nanotags against human ovarian adenocarcinoma cells (
OVCAR-3) are evaluated. The capacity to operate as bright SERS nanotags with precise localization at a single cell level as well as intracellular pH indicators is clearly demonstrated by performing cell imaging under scanning confocal Raman microscopy.


Journal Details

This article was published in the following journal.

Name: Nanotechnology
ISSN: 1361-6528


DeepDyve research library

PubMed Articles [9151 Associated PubMed Articles listed on BioPortfolio]

Application of metallic inks based on nickel-silver core-shell nanoparticles for fabrication of conductive films.

Conductive inks based on nickel nanoparticles (NPs) have attracted much attention as a low-cost replacement for the currently used silver and gold inks for fabrication of printed electronic circuits a...

Fabrication of silver seeds and nanoparticle on core-shell Ag@SiO nanohybrids for combined photothermal therapy and bioimaging.

In this study, two core-shell nanohybrids of different morphologies, namely SiO-coated silver (Ag) with surface-exposed silver seeds (Ag@SiO@Ag) and SiO-coated Ag with surface-exposed Ag nanoparticles...

Fe@(Au/Ag) (n=1,12,54) core-shell nanoparticles as effective drug delivery vehicles for anti-cancer drugs: The computational study.

In this paper, we study magnetic and structural properties of silver and gold-coated iron nanoparticle as novel drug delivery systems for the two commercially famous cancer treatment drugs, using the ...

Catalytic activities of mono- and bi-metal (gold/silver) nanoshells coated gold nanocubes towards catalytic reduction of nitroaromatics.

A new class of core-shell metallic nanostructures with tunable near-surface composition and surface morphology with excellent catalytic activity is reported. Very thin shell of metal nanoassemblies su...

The optimization of methods of synthesis of nickel-silver core-shell nanoparticles for conductive materials.

Nickel-silver core-shell (Ni@Ag) nanoparticles (NPs) were formed in a two-step process: (1) the formation of a dispersion of Ni NPs; and (2) the transmetalation (galvanic displacement) reaction, where...

Clinical Trials [2579 Associated Clinical Trials listed on BioPortfolio]

Topical Silver Nanoparticles for Microbial Activity

Silver nanoparticles are one of most nanoparticles use nowadays in the research area because it has specific physical and chemical properties, in medical fields silver nanoparticles can in...

Topical Application of Silver Nanoparticles and Oral Pathogens in Ill Patients

The purpose of this study is to determine whether oral topic silver nanoparticles are effective to reduce potential pathogen microbial loads in mechanical ventilation patients.

Silver Nanoparticle Investigation for Treating Chronic Sinusitis

Chronic rhinosinusitis is a highly prevalent disease resulting in high economic burden. Effective therapeutic options are needed. Rhinosinusitis-related biofilms are a likely contributor t...

Comparison of Central Venous Catheters With Silver Nanoparticles Versus Conventional Catheters

Bloodstream infections are common in Intensive Care Units (ICUs). The need of a central venous line increases the risk of bacteremia and central venous catheter (CVC) related infections. T...

Assessment of Postoperative Pain After Using Various Intracanal Medication in Patients With Necrotic Pulp

To assess the Postoperative Pain after using Silver Nanoparticles with and without Calcium Hydroxide as an Intracanal Medication in Patients with necrotic pulp (RCT)

Medical and Biotech [MESH] Definitions

Nanoparticles produced from metals whose uses include biosensors, optics, and catalysts. In biomedical applications the particles frequently involve the noble metals, especially gold and silver.

Nanometer range spherical cores of particular semiconductor compounds surrounded by an ultrathin metal shell that is commonly made of gold or silver. This configuration gives the nanoshells highly tunable optical properties. They have potential in biomedicine for diagnosis and therapy.

Nanometer-sized particles that are nanoscale in three dimensions. They include nanocrystaline materials; NANOCAPSULES; METAL NANOPARTICLES; DENDRIMERS, and QUANTUM DOTS. The uses of nanoparticles include DRUG DELIVERY SYSTEMS and cancer targeting and imaging.

The use of silver, usually silver nitrate, as a reagent for producing contrast or coloration in tissue specimens.

Material from which the casting mold is made in the fabrication of gold or cobalt-chromium castings. (Boucher's Clinical Dental Terminology, 4th ed, p168)

Quick Search


DeepDyve research library

Relevant Topics

Women's Health
Women's Health - key topics include breast cancer, pregnancy, menopause, stroke Follow and track Women's Health News on BioPortfolio: Women's Health News RSS Women'...

Radiology is the branch of medicine that studies imaging of the body; X-ray (basic, angiography, barium swallows), ultrasound, MRI, CT and PET. These imaging techniques can be used to diagnose, but also to treat a range of conditions, by allowing visuali...

Cancer Disease
Cancer is not just one disease but many diseases. There are more than 100 different types of cancer. Most cancers are named for the organ or type of cell in which they start - for example, cancer that begins in the colon is called colon cancer; cancer th...

Searches Linking to this Article