Advertisement

Topics

18F-SuPAR: A radiofluorinated probe for non-invasive imaging of DNA damage-dependent Poly (ADP-ribose) Polymerase Activity.

08:00 EDT 11th April 2019 | BioPortfolio

Summary of "18F-SuPAR: A radiofluorinated probe for non-invasive imaging of DNA damage-dependent Poly (ADP-ribose) Polymerase Activity."

Poly (ADP ribose) polymerase (PARP) enzymes generate poly (ADP ribose) post-translational modifications on target proteins for an array of functions centering on DNA and cell stress. PARP isoforms 1 and 2 are critically charged with the surveillance of DNA integrity, and are the first line guardians of the genome against DNA breaks. Here we present a novel probe ([18F]-SuPAR) for non-invasive imaging of PARP-1/2 activity using positron emission tomography (PET). [18F]-SuPAR is a radiofluorinated nicotinamide adenine dinucleotide (NAD) analog that can be recognized by PARP-1/2 and incorporated into the long branched polymers of poly(ADP ribose) (PAR). The measurement of PARP-1/2 activity was supported by a reduction of radiotracer uptake in vivo following PARP-1/2 inhibition with talazoparib treatment, a potent PARP inhibitor currently in Phase III clinical trials, as well as ex vivo co-localization of radiotracer analog and poly (ADP ribose). With [18F]-SuPAR, we were able to map the dose- and time-dependent activation of PARP-1/2 following radiation therapy in breast and cervical cancer xenograft mouse models. Tumor response to therapy was determined by [18F]-SuPAR PET within eight hours of administration of a single dose of radiation equivalent to one round of stereotactic ablative radiotherapy.

Affiliation

Journal Details

This article was published in the following journal.

Name: Bioconjugate chemistry
ISSN: 1520-4812
Pages:

Links

DeepDyve research library

PubMed Articles [19747 Associated PubMed Articles listed on BioPortfolio]

Radiation damage to organic and inorganic specimens in the TEM.

Symptoms of radiation damage are reviewed, followed by a brief description of the three main damage mechanisms: knock-on displacement (predominant in electrically conducting specimens), ionization dam...

Non-Invasive Imaging Biomarker Identifies Small Airway Damage in Severe COPD.

Evidence suggests damage to small airways is a key pathologic lesion in chronic obstructive pulmonary disease (COPD). Computed Tomography (CT) densitometry has been demonstrated to identify emphysema,...

A novel chemiluminescent probe based on 1,2-dioxetane scaffold for imaging cysteine in living mice.

A novel chemiluminescent probe for detection of cysteine (Cys) from other biothiols has been reported by utilizing the excellent chemiluminescent Schaap's adamantylidene-dioxetane scaffold. After care...

Rational Design and Synthesis of A Metalloproteinases-activatable Probe for Dual-modality Imaging of Metastatic Lymph Nodes In Vivo.

Lymphatic metastasis is an important prognostic indicator for cancer progression. It is therefore considerably meaningful to develop molecularly targeted imaging probes for noninvasive and accurate id...

Plasma Soluble Urokinase Plasminogen Activator Receptor Levels in Systemic Lupus Erythematosus Patients.

This study aimed to determine whether plasma soluble urokinase plasminogen activator receptor (suPAR) could serve as an activity biomarker in systemic lupus erythematosus (SLE) patients. suPAR levels ...

Clinical Trials [9564 Associated Clinical Trials listed on BioPortfolio]

suPAR to Guide Antibiotics in Emergency Department

The aim of the current study is to evaluate suPAR - guided medical intervention, consisting of early antibiotic administration at the emergency room for presumed infection and sepsis and e...

Safety Study of FluMist With and Without Ampligen

The purpose of this study is to evaluate FluMist with and without Ampligen in healthy volunteers.

Neoadjuvant Hiltonol® (PolyICLC) for Prostate Cancer

The purpose of this study is to test an approach of stimulating the body's immune system to attack prostate cancer. This study will test injection of a substance polylysine and carboxymeth...

Risk Stratification in Acute Care: The Meaning of suPAR Measurement in Triage

Will clinical outcome for patients be improved if triage in Acute wards and Emergency rooms is supplemented with a prognostic biomarker?

The Role of suPAR Biomarker in Blood Samples of Breast Cancer Patients During and Post Doxorubicin Chemotherapy: Causative vs. Predictor

This study looks to find a causative or predictive aspect of the suPAR biomarker for heart failure in breast cancer patients receiving Doxorubicin drug chemo regimen. suPAR is a circulati...

Medical and Biotech [MESH] Definitions

Diagnostic and therapeutic procedures that are invasive or surgical in nature, and require the expertise of a specially trained radiologist. In general, they are more invasive than diagnostic imaging but less invasive than major surgery. They often involve catheterization, fluoroscopy, or computed tomography. Some examples include percutaneous transhepatic cholangiography, percutaneous transthoracic biopsy, balloon angioplasty, and arterial embolization.

A non-invasive imaging method that uses computed tomographic data combined with specialized imaging software to examine the colon.

A gamma-emitting radionuclide imaging agent used in the evaluation of regional cerebral blood flow and in non-invasive dynamic biodistribution studies and myocardial imaging. It has also been used to label leukocytes in the investigation of inflammatory bowel diseases.

Minimally invasive procedures guided with the aid of magnetic resonance imaging to visualize tissue structures.

Non-invasive imaging of cells that have been labeled non-destructively, such as with nanoemulsions or reporter genes that can be detected by molecular imaging, to monitor their location, viability, cell lineage expansion, response to drugs, movement, or other behaviors in vivo.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Bioinformatics
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Polymerase Chain Reaction (PCR)
PCR (Polymerase Chain Reaction) uses the ability of DNA polymerase (enzymes that create DNA molecules by assembling nucleotides, the building blocks of DNA. These enzymes are essential to DNA replication and usually work in pairs to create two ident...

Diagnostics
A diagnostic test is any kind of medical test performed to aid in the diagnosis or detection of disease. For example: to diagnose diseases to measure the progress or recovery from disease to confirm that a person is free from disease Clin...


Searches Linking to this Article