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EGFR-targeted molecular imaging of colorectal tumors: detection and treatment evaluation of tumors in animal models.

08:00 EDT 11th April 2019 | BioPortfolio

Summary of "EGFR-targeted molecular imaging of colorectal tumors: detection and treatment evaluation of tumors in animal models."

To overcome the problem of overlooking colorectal tumors, a new and highly sensitive modality of colonoscopy is needed. Moreover, it is also important to establish a new modality to evaluate the viable tumor volume in primary lesions of colorectal cancer (CRC) during chemotherapy. Therefore, we performed molecular imaging of colorectal tumors targeting epidermal growth factor receptor (EGFR), which is highly expressed on tumor cells, for evaluating chemotherapeutic efficacy and for endoscopic detection of colorectal adenomas. We first attempted to image 5 CRC cell lines with various levels of EGFR expression using an Alexa Fluor-labeled anti-EGFR monoclonal antibody (AF-EGFR-Ab). A strong fluorescence signal was observed in the cells depending on the level of EGFR expression. When nude mice xenografted with LIM1215 CRC cells, which highly express EGFRs, were intravenously injected with AF-EGFR-Ab, a strong fluorescence signal appeared in the tumor with a high S/N ratio, peaking at 48 h after injection and then gradually decreasing, as revealed using an IVIS Spectrum system. When the xenografted mice were treated with 5-fluorouracil, the fluorescence intensity in the tumor decreased in proportion to the viable tumor cell volume. Moreover, when the colorectum of azoxymethane-treated rats was observed using a thin fluorescent endoscope with AF-EGFR-Ab, all 10 small colorectal adenomas (≤ 3 mm) were detected with a clear fluorescence signal. These preliminary results of animal experiments suggest that EGFR-targeted fluorescent molecular imaging may be useful for quantitatively evaluating cell viability in CRC during chemotherapy, and also for detecting small adenomas using a fluorescent endoscope. This article is protected by copyright. All rights reserved.

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Name: Cancer science
ISSN: 1349-7006
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