Advertisement

Topics

One-pot reactive electrospinning of chitosan/PVA hydrogel nanofibers reinforced by halloysite nanotubes with enhanced fibroblast cell attachment for skin tissue regeneration.

08:00 EDT 27th March 2019 | BioPortfolio

Summary of "One-pot reactive electrospinning of chitosan/PVA hydrogel nanofibers reinforced by halloysite nanotubes with enhanced fibroblast cell attachment for skin tissue regeneration."

In this study, in situ glyoxal crosslinked chitosan/poly (vinyl alcohol) (PVA) hydrogel nanofibers reinforced with halloysite nanotubes (HNT) were prepared by the electrospinning method without needing post-treatment for stabilization of the nanofibers in aqueous media. FTIR spectroscopy approved the formation of acetal bonds between glyoxal and hydroxyl groups of PVA and chitosan. Morphological studies by SEM/EDX and TEM in accordance with XRD patterns proved that HNT was successfully incorporated into the crosslinked chitosan/PVA nanofibers. The crosslinked nanofibers were insoluble in water. Due to the hydrophilic nature of HNT, the swelling of the nanofibers was increased from 272% for crosslinked chitosan/PVA nanofibers to around 400% for the HNT reinforced nanocomposite nanofibers. Comparing to chitosan/PVA nanofibers, the tensile strength of the crosslinked nanocomposite nanofibers was increased to 2.4 and 3.5 fold by incorporation of 3 and 5% HNT, respectively. Presence of HNT in chitosan/PVA nanofibers reduced the contact angle with water and increased the hydrophilicity of HNT-reinforced nanofibers favoring the attachment of fibroblast cells. Cytotoxicity studies by AlamarBlue assay showed that presence of HNT increased the biocompatibility of the nanofibers. It was also concluded that glyoxal can be used safely for crosslinking of chitosan/PVA nanofibers without any cytotoxic effect for fibroblast cells. From the results of this work, HNT reinforced chitosan/PVA nanofibers crosslinked by glyoxal are introduced as promising nanomaterials for skin tissue regeneration.

Affiliation

Journal Details

This article was published in the following journal.

Name: Colloids and surfaces. B, Biointerfaces
ISSN: 1873-4367
Pages: 270-279

Links

DeepDyve research library

PubMed Articles [4687 Associated PubMed Articles listed on BioPortfolio]

Acids 'generally recognized as safe' affect morphology and biocompatibility of electrospun chitosan/polyethylene oxide nanofibers.

Electrospinning of neat chitosan is currently achieved by using strong acids or organic solvents, which limits the use of chitosan nanofibers as biocompatible scaffolds for drug delivery and tissue en...

Activation of natural halloysite nanotubes by introducing lanthanum oxycarbonate nanoparticles via co-calcination for outstanding phosphate removal.

Halloysite nanotubes were activated via co-calcination of halloysite and the precursors of lanthanum oxycarbonate (LO), generating reactive alumina nanoparticles and uniformly anchoring LO nanoparticl...

Investigating the effect of chitosan on hydrophilicity and bioactivity of conductive electrospun composite scaffold for neural tissue engineering.

In this paper, nanofibers containing poly(ε-caprolactone) (PCL), chitosan and polypyrrole (PPy) were fabricated using electrospinning to combine advantages of electrospun nanofibers topography with v...

Single-Step Reactive Electrospinning of Cell-Loaded Nanofibrous Scaffolds as Ready-to-Use Tissue Patches.

A reactive electrospinning strategy is used to fabricate viable and proliferative cell-loaded nanofibrous hydrogel scaffolds in a single step using an all-aqueous approach. In situ-gelling and degrada...

Development and in vitro characterization of chitosan/starch/halloysite nanotubes ternary nanocomposite films.

In this present study, ternary nanocomposite films of chitosan/starch/halloysite nanotubes (ChSHT) were fabricated by solution casting process. The ternary nanocomposite films were characterized by us...

Clinical Trials [792 Associated Clinical Trials listed on BioPortfolio]

Efficacy and Safety of HEP-40 Chitosan for Mild to Moderately Elevated Cholesterol

Chitosan is a natural product that is produced commercially through the deacetylation of chitin, which is found in the exoskeleton of crustaceans. It has been suggested that chitosan has a...

Antimicrobial Effect of Modified Antibiotic Nanofibers for Regenerative Endodontics Procedures

The treatment for immature teeth with pulp necrosis can be carried out through apexification or regenerative endodontics procedures including platelet rich plasma (PRP), platelet rich fibr...

Experimental Study of the Vascular Prosthesis Manufactured by Electrospinning

The purpose of this study is to determine whether the Vascular prosthesis manufactured by Electrospinning is safe with respect to the development of thrombosis.

Chitosan,Chitosan Nanoparticles,and Chlorhexidine Gluconate, as Intra Canal Medicaments in Primary Teeth

Aim of the present study is to assess the in vivo susceptibility of root canal bacteria to Chitosan,Chitosan nanoparticles ,and Chlorhexidine gluconate when used as intra canal medicaments...

A Crossover Trial of Chitosan Oligosaccharide on Post Prandial Glucose Control in Subjects With Normal, IFG and IGT

It is well known that Chitosan oligosaccharide is low molecular weight and water soluble and chitosan oligosaccharide has been shown to reduce blood cholesterol and blood pressure, increas...

Medical and Biotech [MESH] Definitions

Deacetylated CHITIN, a linear polysaccharide of deacetylated beta-1,4-D-glucosamine. It is used in HYDROGEL and to treat WOUNDS.

NANOTUBES formed from cyclic peptides (PEPTIDES, CYCLIC). Alternating D and L linkages create planar rings that self assemble by stacking into nanotubes. They can form pores through CELL MEMBRANE causing damage to cells.

Nanometer-sized tubes composed of various substances including carbon (CARBON NANOTUBES), boron nitride, or nickel vanadate.

Nanometer-sized tubes composed mainly of CARBON. Such nanotubes are used as probes for high-resolution structural and chemical imaging of biomolecules with ATOMIC FORCE MICROSCOPY.

A linear polysaccharide of beta-1->4 linked units of ACETYLGLUCOSAMINE. It is the second most abundant biopolymer on earth, found especially in INSECTS and FUNGI. When deacetylated it is called CHITOSAN.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Wound management
Anything that breaks the skin is a wound because when the skin is broken, there's a risk of germs getting into the body and causing an infection. Follow and track Wound Care News on BioPortfolio: Wound Car...

Assays
An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...


Searches Linking to this Article