Genetic, epigenetic, and developmental toxicity of Chironomus riparius raised in metal-contaminated field sediments: A multi-generational study with arsenic as a second challenge.

08:00 EDT 3rd April 2019 | BioPortfolio

Summary of "Genetic, epigenetic, and developmental toxicity of Chironomus riparius raised in metal-contaminated field sediments: A multi-generational study with arsenic as a second challenge."

Ecotoxicity tests conducted under well-controlled lab conditions often do not reflect the real environmental conditions. To this end, we designed an ecotoxicity test using an aquatic midge, Chironomus riparius, raised in metal-contaminated field sediments (MCFS), which reflect the real environmental conditions, for five consecutive generations (F0-F4) followed by a toxic response to arsenic exposure (as a second challenge). The toxic responses (i.e. DNA damage, DNA methylation, stress response gene expression, and mortality) were compared to those organisms reared in lab sediments (LS). Under the MCFS condition, increased adult emergence was observed for the second and third generations (F1 and F2), while a decreased tendency was evident thereafter (F3 and F4) compared to that of F0. When comparing C. riparius raised in MCFS or LS exposed to arsenic, increased sensitivity (declined survival) was observed in the larvae from F2. However, that tendency was not present in F4 of the MCFS midges, indicating a possible physiological adaptation. Increased DNA damage was observed in the MCFS-exposed organisms (F0, F2, and F4) compared to the those exposed to LS, particularly at F0. Arsenic exposure induced hypermethylation at F0 and, in contrast, hypomethylation at the later generations (F2, F4) in the MCFS-exposed organisms. Global DNA methylation results were supported by the expression of genes involved in enzymatic methylation. Moreover, alterations in oxidative stress related to gene expression showed that significant oxidative stress and perturbation of glutathione reserves occurred under the MCFS and the subsequent arsenic exposure conditions. Overall, our results suggest that multigenerational rearing under MCFS conditions resulted in physiological adaptation of C. riparius to metal exposure, specifically at later generations, which in turn modulated its response to arsenic stress through possible genetic and epigenetic mechanisms.


Journal Details

This article was published in the following journal.

Name: The Science of the total environment
ISSN: 1879-1026
Pages: 789-797


DeepDyve research library

PubMed Articles [16300 Associated PubMed Articles listed on BioPortfolio]

Amitraz toxicity to the midge Chironomus riparius: Life-history and biochemical responses.

Acute and chronic toxicity of the formamidine pesticide amitraz to the midge Chironomus riparius was assessed using conventional ecotoxicological tests and biochemical approaches (biomarkers). Amitraz...

Enantioselective mixture toxicity of the azole fungicide imazalil with the insecticide α-cypermethrin in Chironomus riparius: Investigating the importance of toxicokinetics and enzyme interactions.

The fungicide imazalil is a chiral compound with one R- and one S-enantiomer. Enantiomers, while having the same chemical properties, can differ in their biological activity expressed as efficacy/toxi...

Chironomus riparius exposure to field-collected contaminated sediments: From subcellular effect to whole-organism response.

The toxicity of three field-collected sediments differentially contaminated with pesticides, heavy metals, phtalates and polycyclic aromatic hydrocarbons (PAHs), was assessed in Chironomus riparius. F...

Ecotoxicity assessment of boreal lake sediments affected by metal mining: Sediment quality triad approach complemented with metal bioavailability and body residue studies.

There are several methods for studying metal-contaminated freshwater sediments, but more information is needed on which methods to include in ecological risk assessment. In this study, we compliment t...

Effects at molecular level of multi-walled carbon nanotubes (MWCNT) in Chironomus riparius (DIPTERA) aquatic larvae.

Nowadays, due to the physical, chemical, electrical, thermal and mechanical properties of carbon nanotubes (CNT), its have been currently incorporated into biomedical products and they are employed in...

Clinical Trials [5616 Associated Clinical Trials listed on BioPortfolio]

Can Epimutations be Inherited? How to Manage Patients With Imprinting-related Diseases Who Wish to Become Parents

Like genetic mutations, DNA methylation anomalies or epimutations can disrupt gene expression and lead to human diseases. However, unlike genetic mutations, epimutations can in theory be ...

Translating Evidence Based Developmental Screening Into Pediatric Primary Care

The purpose of this study is to examine the feasibility, acceptability and effectiveness of implementing the AAP's recommendation that clinicians provide developmental surveillance at all ...

Metal Ion Release From an FDA-Approved Metal-on-Metal Total Hip Replacement Implant

This is a multi-center, randomized study to compare blood and urine cobalt, chromium, and titanium ion levels of a metal-on-metal articular bearing coupled with two different head sizes to...

A Multicentre Trial to Compare Two Articulating Bearing Surfaces as Used in Cementless Primary Hip Arthroplasty

The aim of this study is to demonstrate the non-inferiority of the ceramic-on-metal articulation using large diameter bearings (38mm to 60mm) compared to the metal on metal articulation us...

Investigation of Wet Cupping Therapy on Heavy Metal Levels of Steel Industry Workers

This interventional study investigates the efficacy of Wet cupping therapy on heavy metal levels.The levels of the selected heavy metals will be measured in the blood samples obtained from...

Medical and Biotech [MESH] Definitions

A class II histone deacetylase that removes acetyl groups from N-terminal LYSINES of HISTONE H2A; HISTONE H2B; HISTONE H3; and HISTONE H4. It plays a critical role in EPIGENETIC REPRESSION and regulation of GENETIC TRANSCRIPTION, as well as CELL MOTILITY through deacetylation of TUBULIN. It also targets misfolded proteins for clearance by AUTOPHAGY when MOLECULAR CHAPERONE-mediated folding is overwhelmed.

Production of new arrangements of DNA by various mechanisms such as assortment and segregation, CROSSING OVER; GENE CONVERSION; GENETIC TRANSFORMATION; GENETIC CONJUGATION; GENETIC TRANSDUCTION; or mixed infection of viruses.

Dysfunctions in the metabolism of metals resulting from inborn genetic mutations that are inherited or acquired in utero.

The turning off of GENETIC TRANSCRIPTION in certain regions of CHROMATIN without changes in the DNA sequence. Typically epigenetic repression is a way that developmental changes are programmed at the cellular level.

A family of nonbiting midges, in the order DIPTERA. Salivary glands of the genus Chironomus are used in studies of cellular genetics and biochemistry.

Quick Search


DeepDyve research library

Relevant Topics

The development and maintenance of an organism is orchestrated by a set of chemical reactions that switch parts of the genome off and on at strategic times and locations. Epigenetics is the study of these reactions and the factors that influence them. ...

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Stress is caused by your perception of situations around you and then the reaction of your body to them. The automatic stress response to unexpected events is known as 'fight or flight'. Discovered by Walter Cannon in 1932, it is the release of h...

Searches Linking to this Article