Applications of multiplex genome editing in higher plants.

08:00 EDT 9th April 2019 | BioPortfolio

Summary of "Applications of multiplex genome editing in higher plants."

Multiplex genome editing involves the simultaneous targeting of multiple related or unrelated targets. The latter is most straightforward using the CRISPR/Cas9 system because multiple gRNAs can be delivered either as independent expression cassettes with their own promoters or as polycistronic transcripts processed into mature gRNAs by endogenous or introduced nucleases. Multiplex genome editing in plants initially focused on input traits such as herbicide resistance, but has recently expanded to include hormone biosynthesis and perception, metabolic engineering, plant development and molecular farming, with more than 100 simultaneous targeting events reported. Usually the coding region is targeted but recent examples also include promoter modifications to generate mutants with varying levels of gene expression.


Journal Details

This article was published in the following journal.

Name: Current opinion in biotechnology
ISSN: 1879-0429
Pages: 93-102


DeepDyve research library

PubMed Articles [17510 Associated PubMed Articles listed on BioPortfolio]

A Single Transcript CRISPR-Cas9 System for Multiplex Genome Editing in Plants.

The CRISPR-Cas9 system has been widely adopted in genome editing. By changing the 20 bp guide sequence, it can easily edit any sequence adjacent to a protospacer adjacent motif (PAM) in a genome. Mul...

Cas9-NG greatly expands the targeting scope of genome-editing toolkit by recognizing NG and other atypical PAMs in rice.

The CRISPR technologies enabling precise genome manipulation are valuable for the gene function study and molecular crop breeding. However, the requirement of a protospacer adjacent motif (PAM), such ...

Efficient CRISPR-Cas9 mediated multiplex genome editing in yeasts.

The thermotolerant methylotrophic yeast has been regarded as an important organism for basic research and biotechnological applications. It is generally recognized as an efficient and safe cell facto...

A Multiplexed CRISPR/Cas9 Editing System Based on the Endogenous tRNA Processing.

The CRISPR-Cas9 system has become a powerful and popular tool for genome editing due to its efficiency and simplicity. Multiplex genome editing is an important feature of the CRISPR-Cas9 system and re...

Enhanced genome editing in rice using single transcript unit CRISPR-LbCpf1 systems.

A variety of sequence-specific nucleases (SSNs) have been successfully used for plant genome editing. Clustered regularly interspaced short palindromic repeat (CRISPR)-CRISPR-associated (Cas) systems,...

Clinical Trials [2302 Associated Clinical Trials listed on BioPortfolio]

Ascending Dose Study of Genome Editing by the Zinc Finger Protein (ZFP) Therapeutic SB-318 in Subjects With MPS I

The purpose of the study is to evaluate the safety, tolerability and effect on leukocyte and plasma iduronidase (IDUA) enzyme activity of ascending doses of SB-318. SB-318 is an intravenou...

Ascending Dose Study of Genome Editing by the Zinc Finger Nuclease (ZFN) Therapeutic SB-913 in Subjects With MPS II

The purpose of the study is to evaluate the safety, tolerability and effect on leukocyte and plasma Iduronate 2-Sulfatase (IDS) enzyme activity of ascending doses of SB-913. SB-913 is an i...

Multiplex Analysis of Circulating Tumor DNA

Lung cancer is the leading cause of cancer-related death in China. This study will explore the applications of multiplex analysis of circulating tumor DNA biomarkers for diagnosis and surv...

Multiplex Laser vs. PDL (Pulsed Dye Laser ) in the Treatment of Surgical Scars

This study aims to determine if the 595nm Pulsed dye laser, the combined 595/1064nm Multiplex laser or no treatment results in a better outcome in the quality of the surgical scar using th...

Paediatric Infections Point-Of-Care

This study aims to identify the aetiology of childhood meningitis in Southwestern Uganda and develop and evaluate new methods for point-of-care diagnosis of childhood meningitis in a low-i...

Medical and Biotech [MESH] Definitions

Genetic engineering techniques that involve DNA REPAIR mechanisms for incorporating site-specific modifications into a cell's genome.

PLANTS, or their progeny, whose GENOME has been altered by GENETIC ENGINEERING.

An APOBEC deaminase catalytic subunit of the apolipoprotein B (APOB) MESSENGER RNA (mRNA) editing enzyme complex that is involved in post-transcriptional editing of a CAA codon for GLYCINE to a UAA STOP CODON in the ApoB mRNA. It also functions in CGA (ARGININE) to UGA STOP CODON editing of NEUROFIBROMIN 1 mRNA and EPIGENETIC PROCESSES.

A disorder characterized by multiple, wide spread cutaneous cysts that often become inflamed and rupture. It is caused by the same mutations in the gene coding for KRT-17 that are causative mutations for Pachyonychia congenita, Type 2. Natal teeth involvement is sometimes associated with steatocystoma multiplex.

Plants whose roots, leaves, seeds, bark, or other constituent possess therapeutic, tonic, purgative, or other pharmacologic activity when administered to higher animals.

Quick Search


DeepDyve research library

Relevant Topics

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Endocrine Disorders
Endocrine disorders are grouped into two categories: hormone imbalance - when a gland produces too much or too little of an endocrine hormone development of lesions (such as nodules or tumors) in the endocrine system, which may or may not affect...

Gene Expression
The process of gene expression is used by eukaryotes, prokaryotes, and viruses to generate the macromolecular machinery for life. Steps in the gene expression process may be modulated, including the transcription, RNA splicing, translation, and post-tran...

Searches Linking to this Article