Xenin-25 induces anion secretion by activating non-cholinergic secretomotor neurons in the rat ileum.

08:00 EDT 12th April 2019 | BioPortfolio

Summary of "Xenin-25 induces anion secretion by activating non-cholinergic secretomotor neurons in the rat ileum."

Xenin-25 is a neurotensin-like peptide that is secreted by enteroendocrine cells in the small intestine. Xenin-8 is reported to augment duodenal anion secretion by activating afferent neural pathways. The intrinsic neuronal circuits mediating the Xenin-25-induced anion secretion were characterized using Ussing chambered mucosa-submucosa preparation from the rat ileum. Serosal application of Xenin-25 increased the short-circuit current ( Isc) in a concentration-dependent manner. The responses were abolished by the combination of Cl-free and HCO-free solutions. The responses were almost completely blocked by TTX (10 M), but not by atropine (10 M) or hexamethonium (10 M). The selective antagonists for NTSR1, NK1, VPAC1, VPAC2 and capsaicin, but not 5-HT, 5-HT, NTSR2 and A803467, inhibited the responses to Xenin-25. The expression of VIP receptors in rat ileum was examined using RT-PCR. The Vipr1 PCR products were detected in the submucosal plexus and mucosa. Immunohistochemical staining showed the colocalization of NTSR1 and NK1 with SP- and calbindin-immunoreactive neurons in submucosal plexus, respectively. In addition, NK1 was also colocalized with non-cholinergic VIP secretomotor neurons. The present study indicates that Xenin-25-induced Cl/HCO secretion is involved in NTSR1 activation on intrinsic and extrinsic afferent neurons, followed by the release of SP and subsequent activation of NK1 expressed on non-cholinergic VIP secretomotor neurons. Finally, the secreted VIP may activate VPAC1 on epithelial cells to induce Cl/HCO secretion in the rat ileum. Activation of VIP-positive non-cholinergic secretomotor neurons by IPANs and extrinsic afferent neurons by postprandially released Xenin-25 may account for most of the neurogenic secretory response induced by Xenin-25.


Journal Details

This article was published in the following journal.

Name: American journal of physiology. Gastrointestinal and liver physiology
ISSN: 1522-1547


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