Label-free proteomic analysis reveals parasite-specific protein alterations in macrophages following Leishmania amazonensis, Leishmania major or Leishmania infantum infection.

08:00 EDT 12th April 2019 | BioPortfolio

Summary of "Label-free proteomic analysis reveals parasite-specific protein alterations in macrophages following Leishmania amazonensis, Leishmania major or Leishmania infantum infection."

Leishmania is an obligate intracellular parasite known to modulate the host cell to survive and proliferate. However, the complexity of host-parasite interactions remains unclear. Also, the outcome of the disease has been recognized to be species-specific and dependent on the host`s immune responses. Proteomics has emerged as a powerful tool to investigate the host-pathogen interface, allowing us to deepen our knowledge about infectious diseases. Quantification of the relative amount of proteins in a sample can be achieved using label-free proteomics, and, for the first time, we have used it to quantify Leishmania-specific protein alterations in macrophages. Protein extracts were obtained and digested, and peptides were identified and quantified using nano-LC coupled with tandem mass spectrometry analyses. Protein expression was validated by Western blot analysis. Integrated Proteomics Pipeline was used for peptide/protein identification and for quantification and data processing. Ingenuity Pathway Analysis was used for network analysis. In this work, we investigated how this intracellular parasite modulates protein expression on host macrophage by comparing three different Leishmania species: L. amazonensis, one of the causative agents of cutaneous disease in the Amazon region; L. major, another causative agent of cutaneous leishmaniasis in Africa, the Middle East, China, India; L. infantum, the causative agent of visceral leishmaniasis affecting humans and dogs in Latin America; and lipopolysaccharide stimulated macrophages as an in vitro inflammation model. Our results revealed that Leishmania infection downregulates apoptosis pathways while upregulates the activation of phagocytes/leukocytes and lipid accumulation.


Journal Details

This article was published in the following journal.

Name: ACS infectious diseases
ISSN: 2373-8227


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