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A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding-based biomonitoring.

08:00 EDT 12th April 2019 | BioPortfolio

Summary of "A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding-based biomonitoring."

This study summarizes results of a DNA barcoding campaign on German Diptera, involving analysis of 45,040 specimens. The resultant DNA barcode library includes records for 2,453 named species comprising a total of 5,200 BINs, including 2,700 CO1 haplotype clusters without species level assignment, so called "dark taxa". Overall, 88 out of 117 families (75%) recorded from Germany were covered, more than 50% of the 9,544 known species of Diptera Germany. Until now, most of these families, especially the most diverse ones, have been taxonomically inaccessible because of the lack of specialists. By contrast, within a few years this study provided an intermediate taxonomic system for half of the German Dipteran fauna, which will provide a useful foundation for subsequent detailed, integrative taxonomic studies. Using DNA extracts derived from bulk collections made by Malaise traps, we further demonstrate that species delineation using BINs and OTUs constitutes an effective method for biodiversity studies using DNA metabarcoding. As the reference libraries will continue to grow, and gaps in the species catalogue will be filled, BIN lists assembled by metabarcoding will gain incremental taxonomic resolution. The present study has three main goals: (1) provide a DNA barcode library for 5,200 BINs of Diptera; (2) demonstrate by the example of bulk extractions from a Malaise Trap experiment, that DNA barcode clusters, labelled with globally unique identifiers (such as OTUs and/or BINs), provide a pragmatic, accurate solution to the 'taxonomic impediment'; and (3) demonstrate that interim names based on BINs and OTUs obtained through metabarcoding is an effective method for studies on species-rich groups that are usually neglected in biodiversity research projects because of their unresolved taxonomy. This article is protected by copyright. All rights reserved.

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This article was published in the following journal.

Name: Molecular ecology resources
ISSN: 1755-0998
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Medical and Biotech [MESH] Definitions

An order of the class Insecta. Wings, when present, number two and distinguish Diptera from other so-called flies, while the halteres, or reduced hindwings, separate Diptera from other insects with one pair of wings. The order includes the families Calliphoridae, Oestridae, Phoridae, Sarcophagidae, Scatophagidae, Sciaridae, SIMULIIDAE, Tabanidae, Therevidae, Trypetidae, CERATOPOGONIDAE; CHIRONOMIDAE; CULICIDAE; DROSOPHILIDAE; GLOSSINIDAE; MUSCIDAE; TEPHRITIDAE; and PSYCHODIDAE. The larval form of Diptera species are called maggots (see LARVA).

A suborder of insects which belong to the order DIPTERA. They include mosca, mosquito, gnats, black flies, true flies and long-horned flies.

A family of the order DIPTERA that includes the TSETSE FLIES. These flies occur only in Africa.

Insects of the order DIPTERA, suborder NEMATOCERA. They include mosquitoes, gnats, black flies, and true flies.

Family of flies in the order DIPTERA, commonly known as flesh flies. They lay their eggs in dead or decaying matter or open wounds.

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