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Evaluation of dried blood spots for hepatitis B and D serology and nucleic acid testing.

08:00 EDT 12th April 2019 | BioPortfolio

Summary of "Evaluation of dried blood spots for hepatitis B and D serology and nucleic acid testing."

Areas with the highest burden of hepatitis B virus (HBV) infection are often low-middle-income countries with limited access to diagnosis due to isolation, affordability and/or feasibility. Dried blood spots (DBS) provide an alternative for remote areas where collection and transportation of serum is impractical. In this study the application of DBS for serological and molecular detection of HBV and hepatitis D virus (HDV) was evaluated. HBsAg was detected in 87 of 91 (95.6%) DBS. 17/21 (81%) had detectable HBeAg and 52/71 (73.2%) were anti-HBe positive. Anti-HD was detectable in 11/12 (91.6%) spiked control DBS after an initial failure to detect in patient DBS. HBV DNA was detected from 50/70 (71.4%) DBS with serum loads greater than 200IU/mL in an in-house assay and 18/24 (75%) DBS with loads exceeding 389IU/mL in a commercial assay. Using linear regression, HBV DNA loads from DBS were able to predict serum loads in 46 of 50 (92%) samples to within 1 log of actual serum load. HDV RNA was detected in 42 of 47 (89%) DBS with serum levels greater than 7,200IU/mL. DBS are recommended for diagnosis of HBV, monitoring and detection of high loads in pregnant women where peripheral blood testing remains unfeasible. Detection of HDV RNA from DBS may prove useful in endemic areas. This article is protected by copyright. All rights reserved.

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This article was published in the following journal.

Name: Journal of medical virology
ISSN: 1096-9071
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