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Modeling of aluminum-induced malate secretion by pharmacological approach in Arabidopsis: involvement of lipid signaling.

08:00 EDT 12th April 2019 | BioPortfolio

Summary of "Modeling of aluminum-induced malate secretion by pharmacological approach in Arabidopsis: involvement of lipid signaling."

To identify the upstream signaling of aluminum-induced malate secretion through aluminum-activated malate transporter 1 (AtALMT1), a pharmacological assay using inhibitors of human signal transduction pathways was performed. Early aluminum-induced transcription of AtALMT1 and other aluminum-responsive genes was significantly suppressed by phosphatidylinositol 4-kinase (PI4K) and phospholipase C (PLC) inhibitors, indicating that the PI4K-PLC metabolic pathway activates early aluminum-signaling. Inhibitors of phosphatidylinositol 3-kinase (PI3K) and PI4K reduced aluminum-activated malate transport by AtALMT1, suggesting that both the PI3K and PI4K metabolic pathways regulate this process. These results were validated using T-DNA insertion mutants of PI4K and PI3K-RNAi lines. A human protein kinase inhibitor, putatively inhibiting homologous calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) and/or Ca-dependent protein kinase (CDPK) in Arabidopsis, suppressed late-phase aluminum-induced expression of AtALMT1, which was concomitant with the induction of AtALMT1 repressor, WRKY46 and suppression of AtALMT1 activator, Calmodulin-binding transcription activator 2 (CAMTA2). In addition, a human deubiquitinase inhibitor suppressed aluminum-activated malate transport, suggesting that deubiquitinases can regulate this process. We also found a reduction of aluminum-induced citrate secretion in tobacco by applying inhibitors of PI3K and PI4K. Taken all, our results indicated that phosphatidylinositol metabolism regulates organic acid secretion in plants under aluminum stress.

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This article was published in the following journal.

Name: Journal of experimental botany
ISSN: 1460-2431
Pages:

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Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.

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