Modeling of aluminum-induced malate secretion by pharmacological approach in Arabidopsis: involvement of lipid signaling.

08:00 EDT 12th April 2019 | BioPortfolio

Summary of "Modeling of aluminum-induced malate secretion by pharmacological approach in Arabidopsis: involvement of lipid signaling."

To identify the upstream signaling of aluminum-induced malate secretion through aluminum-activated malate transporter 1 (AtALMT1), a pharmacological assay using inhibitors of human signal transduction pathways was performed. Early aluminum-induced transcription of AtALMT1 and other aluminum-responsive genes was significantly suppressed by phosphatidylinositol 4-kinase (PI4K) and phospholipase C (PLC) inhibitors, indicating that the PI4K-PLC metabolic pathway activates early aluminum-signaling. Inhibitors of phosphatidylinositol 3-kinase (PI3K) and PI4K reduced aluminum-activated malate transport by AtALMT1, suggesting that both the PI3K and PI4K metabolic pathways regulate this process. These results were validated using T-DNA insertion mutants of PI4K and PI3K-RNAi lines. A human protein kinase inhibitor, putatively inhibiting homologous calcineurin B-like protein (CBL)-interacting protein kinase (CIPK) and/or Ca-dependent protein kinase (CDPK) in Arabidopsis, suppressed late-phase aluminum-induced expression of AtALMT1, which was concomitant with the induction of AtALMT1 repressor, WRKY46 and suppression of AtALMT1 activator, Calmodulin-binding transcription activator 2 (CAMTA2). In addition, a human deubiquitinase inhibitor suppressed aluminum-activated malate transport, suggesting that deubiquitinases can regulate this process. We also found a reduction of aluminum-induced citrate secretion in tobacco by applying inhibitors of PI3K and PI4K. Taken all, our results indicated that phosphatidylinositol metabolism regulates organic acid secretion in plants under aluminum stress.


Journal Details

This article was published in the following journal.

Name: Journal of experimental botany
ISSN: 1460-2431


DeepDyve research library

PubMed Articles [22016 Associated PubMed Articles listed on BioPortfolio]

Entry and Deposit of Aluminum in the Brain.

Aluminum, as a known neurotoxicant, contributes to cognitive dysfunction and may contribute to Alzheimer's disease. The important reason is that aluminum can enter and be deposited in the brain. There...

Calcium signaling and secretory granule pool dynamics underlie biphasic insulin secretion and its amplification: experiments and modeling.

Glucose-stimulated insulin secretion from pancreatic β-cells is controlled by a triggering pathway culminating in calcium influx and regulated exocytosis of secretory granules, and a less understood ...

Endophyte infection enhances accumulation of organic acids and minerals in rice under Pb stress conditions.

This study aimed to assess the effects of endophyte infection on Pb accumulation, organic acids (OAs) and mineral element contents by comparing endophyte-uninfected (E-) and endophyte-infected (E+) ri...

Aluminum-Induced Electrophysiological Variation, Synaptic Plasticity Impairment, and Related Mechanism.

Aluminum, an environmentally abundant non-redox trivalent cation, has long been reported to alter blood-brain barrier and gets deposited in different regions of the brain. Many reports strongly indica...

Occupational Exposure to Aluminum and Cognitive Impairment.

Aluminum industry has been producing more than a century of history. Aluminum is a light metal which is widely used in industrial applications. Occupational aluminum workers are an important source fo...

Clinical Trials [6844 Associated Clinical Trials listed on BioPortfolio]

Sunitinib Malate in Treating Patients With Kidney Cancer

RATIONALE: Sunitinib malate may stop the growth of tumor cells by blocking some of the enzymes needed for cell growth and by blocking blood flow to the tumor. PURPOSE: This clinical trial...

Impact of Aluminum on Sperm DNA Quality

Between 1950 and 2013, aluminum production was multiplied by thirty in the world. Today, men's exposure to aluminum, including food products, cosmetics, air and water contamination, and a ...

Cabozantinib-s-malate or Sunitinib Malate in Treating Patients With Previously Untreated Locally Advanced or Metastatic Kidney Cancer

This randomized phase II trial studies how well cabozantinib-s-malate works compared to sunitinib malate in treating patients with previously untreated kidney cancer that has spread to nea...

Cabozantinib-S-Malate in Treating Patients With Recurrent or Metastatic Endometrial Cancer

This phase II trial studies how well cabozantinib-s-malate works in treating patients with recurrent or metastatic endometrial cancer. Cabozantinib-s-malate may stop the growth of tumor ce...

Cabozantinib-S-Malate in Treating Patients With Advanced Solid Tumors and Human Immunodeficiency Virus

This phase I trial studies the side effects and best dose of cabozantinib-s-malate in treating patients with advanced solid tumors and human immunodeficiency virus. Cabozantinib-s-malate m...

Medical and Biotech [MESH] Definitions

Proteins that originate from plants species belonging to the genus ARABIDOPSIS. The most intensely studied species of Arabidopsis, Arabidopsis thaliana, is commonly used in laboratory experiments.

An enzyme that catalyzes the conversion of (S)-malate and NAD+ to oxaloacetate and NADH. EC

A statistical tool for detecting and modeling gene-gene interactions. It is a non-parametric and model-free approach.

A light-activated enzyme that catalyzes the oxidation of (S)-malate to OXALOACETATE. It is involved in PYRUVATE metabolism and CARBON fixation.

An important enzyme in the glyoxylic acid cycle which reversibly catalyzes the synthesis of L-malate from acetyl-CoA and glyoxylate. This enzyme was formerly listed as EC

Quick Search


DeepDyve research library

Relevant Topics

An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity.  This can be a drug or biochemical substance or a cell in an organism or organic sample. ...

Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...

Searches Linking to this Article