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The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family.

08:00 EDT 21st March 2019 | BioPortfolio

Summary of "The crystal structure of the CopC protein from Pseudomonas fluorescens reveals amended classifications for the CopC protein family."

The bacterial CopC family of proteins are periplasmic copper binding proteins that act in copper detoxification. These proteins contain Cu(I) and/or Cu(II) binding sites, with the family that binds Cu(II) only the most prevalent, based on sequence analyses. Here we present three crystal structures of the CopC protein from Pseudomonas fluorescens (Pf-CopC) that include the wild type protein bound to Cu(II) and two variant proteins, where Cu(II) coordinating ligands were mutated, in Cu-free states. We show that the Cu(II) atom in Pf-CopC is coordinated by two His residues, an Asp residue and the N-terminus of the protein (therefore a 3N + O site). This coordination structure is consistent with all structurally characterized proteins from the CopC family to date. Structural and sequence analyses of the CopC family allow a relationship between protein sequence and the Cu(II) binding affinity of these proteins to be proposed.

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Journal Details

This article was published in the following journal.

Name: Journal of inorganic biochemistry
ISSN: 1873-3344
Pages: 194-200

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