An ultrasensitive electrochemiluminescence biosensor for MicroRNA detection based on luminol-functionalized Au NPs@ZnO nanomaterials as signal probe and dissolved O as coreactant.

08:00 EDT 6th April 2019 | BioPortfolio

Summary of "An ultrasensitive electrochemiluminescence biosensor for MicroRNA detection based on luminol-functionalized Au NPs@ZnO nanomaterials as signal probe and dissolved O as coreactant."

In this work, the ZnO nanostars with excellent catalytic performance were firstly used as the coreaction accelerator of luminol-O system to construct a biosensor for ultrasensitively detecting microRNA-21 (miRNA-21) in cancer cells. Specifically, ZnO nanostars could expedite the reduction of dissolved O, generating more reactive oxygen species (ROSs) to extremely promote electrochemiluminescence (ECL) luminous efficiency of luminol. Thus luminol-functionalized Au NPs@ZnO (L-Au NPs@ZnO) nanomaterials were employed as signal probe to fabricate sensing nano-platform for achieving significant ECL emission as "signal on" state. Moreover, upon the addition of a tiny minority of target miRNA-21, massive ferrocene (Fc) could be immobilized on the sensing interface through hybridization chain reaction (HCR) triggered-DNA dendrimers self-assembly, in which Fc consumed dissolved O for prominently quenching the ECL emission of signal probe and then reached a "signal off" state. As a result, the biosensor performed a good linearity in 100 aM - 100 pM and a low limit of detection (LOD) down to 18.6 aM. In general, this work utilized a new coreaction accelerator as an efficient amplification approach for ultrasensitively detecting target analyses, providing a promising approach in luminol-centric ECL bioanalysis fields.


Journal Details

This article was published in the following journal.

Name: Biosensors & bioelectronics
ISSN: 1873-4235
Pages: 8-13


DeepDyve research library

PubMed Articles [19119 Associated PubMed Articles listed on BioPortfolio]

An enzyme-free electrochemiluminescence biosensor for ultrasensitive assay of Group B Streptococci based on self-enhanced luminol complex functionalized CuMn-CeO nanospheres.

Herein, a novel and pragmatic electrochemiluminescence (ECL) biosensing method was developed for ultrasensitive and specific detection of Group B Streptococci (GBS) by combining self-enhanced luminol ...

A novel ultrasensitive electrochemiluminescence biosensor for glutathione detection based on poly-L-lysine as co-reactant and graphene-based poly(luminol/aniline) as nanoprobes.

In this work, an ultrasensitive electrochemiluminescence (ECL) biosensor was constructed using poly-L-lysine (PLL) as a novel co-reactant of luminol and poly(luminol/aniline) nanorods loaded reduced g...

Highly-efficient luminol immobilization approach and exponential strand displacement reaction based electrochemiluminescent strategy for monitoring microRNA expression in cell.

This work used 3,4,9,10-perylenetetracarboxylic acid-luminol composite (PTCA-luminol) as signal tag with improved ECL signal and applied cruciform DNA structure mediated exponential strand displacemen...

Ultrasensitive amyloid-β proteins detection based on curcumin conjugated ZnO nanoparticles quenching electrochemiluminescence behavior of luminol immobilized on Au@MoS/BiS nanorods.

This work reported curcumin quenched electrochemiluminescence (ECL) behavior of luminol with HO as coreactant. To achieve excellent ECL signal, luminol was anchored on Au functionalized MoS/BiS nanoro...

Electrochemiluminescence resonance energy transfer biosensor between the glucose functionalized MnO and g-CN nanocomposites for ultrasensitive detection of concanavalin A.

An electrochemiluminescence (ECL) analytical platform was initially proposed based on the electrochemiluminescence resonance energy transfer (ECL-RET) mechanism for ultrasensitive detection of Concana...

Clinical Trials [4869 Associated Clinical Trials listed on BioPortfolio]

Detection of MicroRNA-25 in the Diagnosis of Pancreatic Cancer

Pancreatic cancer represents the most lethal of the common malignancies, with a 5-year survival rate of less than 5%. For patients who, when are diagnosed of pancreatic cancer, are eligibl...

Detection of Gastrointestinal Bleeding in Intensive Care Patients Via Biosensor Watch

The purpose of this study to improve methods of monitoring and diagnosing gastrointestinal bleeding via the E4 wristband, a biosensor watch.

OstiSense Biosensor in Bruxism Reduction - A Clinical Study

The overall objective of the clinical studies with the OstiSense biosensor tool will be to evaluate whether the use of the OstiSense biosensor tool significantly reduces the number of slee...

microRNA of Human Epidermal Growth Factor Receptor 2 (HER2)Positive Patient Treated With Herceptin

This projective observational study is planned to enroll more than 300 advanced breast cancer patients, who were proved as Her-2 positive using fluorescence in situ hybridization (FISH) an...

Physiopathological and Therapeutic Value of microRNA in the Progression of Carotid Artery Plaques: Carotid Protocol and microRNA

Molecular analysis of the atheroma plaque. Screening for a novel biomarker of carotid status.

Medical and Biotech [MESH] Definitions

Detection of drugs that have been abused, overused, or misused, including legal and illegal drugs. Urine screening is the usual method of detection.

5-Amino-2,3-dihydro-1,4-phthalazinedione. Substance that emits light on oxidation. It is used in chemical determinations.

Therapeutic cult concerned with intangible energies surrounding the living body and based on the detection of these intrinsic radiations by dowsing, or divining, or the use of more elaborate instruments (radionics).

Ascertaining of deception through detection of emotional disturbance as manifested by changes in physiologic processes usually using a polygraph.

A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

Quick Search


DeepDyve research library

Relevant Topics

MicroRNAs (miRNAs)
A microRNA (abbreviated miRNA) is a small non-coding RNA molecule (containing about 22 nucleotides) found in plants, animals, and some viruses.  Key findings: miRNA is involved in the normal functioning of eukaryotic cells, so has dysregulation...

  Bladder Cancer Brain Cancer Breast Cancer Cancer Cervical Cancer Colorectal Head & Neck Cancers Hodgkin Lymphoma Leukemia Lung Cancer Melanoma Myeloma Ovarian Cancer Pancreatic Cancer ...

Searches Linking to this Article