Down-regulation of corticosteroid receptor in leucocytes of stressed rainbow trout.

08:00 EDT 10th April 2019 | BioPortfolio

Summary of "Down-regulation of corticosteroid receptor in leucocytes of stressed rainbow trout."

The relationship between stress and immunosuppression was investigated in peripheral blood leucocytes (PBL) in rainbow trout, with reference to corticosteroid receptor (CR) expression and responses to cortisol- and/or lipopolysaccharide (LPS)-administration. Confinement stress in shallow water resulted in a sustained elevation of plasma cortisol, whereas lysozyme and immunoglobin levels were suppressed. Significant increases in mRNA levels of caspase-6 and insulin-like growth factor (IGF)-I were observed in PBL isolated from stressed fish. Confinement stress also suppressed proinflammatory cytokine, interleukin (IL)-1β, expression in PBL. There were decreasing tendencies for the mRNA levels of CRs in PBL of stressed fish. In-vitro treatment of cortisol and LPS on isolated PBL from unstressed trout increased both IL-1 β and CR mRNA expression. However, in PBL from stressed fish, cortisol and LPS treatment increased IL-1 β but not CR mRNA levels. Proliferative activities estimated as in-vitro incorporation of bromodeoxyuridine (BrdU) were decreased by cortisol in PBL from the unstressed and stressed fish groups; however, LPS-stimulated proliferation was observed only in the unstressed fish. Ratios of apoptotic PBL quantified as cell fragmentation using an automated cell counter were increased by cortisol in both groups; however, LPS-stimulated apoptosis was observed only in the stressed fish. Our study reveals cortisol has immune-suppressive effects in stressed fish, irrespective of CR down-regulation and desensitization. The complexity of immune-endocrine interaction is shown by the stress-induced attenuation of LPS effects.


Journal Details

This article was published in the following journal.

Name: General and comparative endocrinology
ISSN: 1095-6840


DeepDyve research library

PubMed Articles [10324 Associated PubMed Articles listed on BioPortfolio]

A pentavalent vaccine for rainbow trout in Danish aquaculture.

Mariculture in Denmark is based on production of rainbow trout grown two years in fresh water followed by one growth season in sea cages. Although the majority of rainbow trout are vaccinated against ...

Local regulation of immune genes in rainbow trout (Oncorhynchus mykiss) naturally infected with Flavobacterium psychrophilum.

Flavobacterium psychrophilum is the etiological agent of bacterial cold water disease (BCWD), also referred to as rainbow trout fry syndrome (RTFS), a disease with great economic impact in salmonid aq...

Cadmium disrupts melanocortin 2 receptor signaling in rainbow trout.

Cadmium is an endocrine disruptor and inhibits corticosteroid production, but the mechanisms are far from clear. We tested the hypothesis that sublethal exposure to environmentally realistic levels of...

An in-situ method for real-time discriminating salmons and rainbow trout without sample preparation using iKnife and rapid evaporative ionization mass spectrometry based lipidomics.

The domestic rainbow trout producers issued a standard with an aquatic association that classified rainbow trout as salmon, which raised the concern of consumers on the fish parasites infection. Herei...

Pck-ing up steam: Widening the salmonid gluconeogenic gene duplication trail.

Rainbow trout have, as salmonid fish species, undergone sequential genome duplication events in their evolutionary history. In addition to a teleost-specific whole genome duplication approximately 320...

Clinical Trials [3767 Associated Clinical Trials listed on BioPortfolio]

Acute Single Meal Effects of Trout on Cardiovascular Risk Markers and Plasma Proteome

The acute effects of farmed rainbow trout, fed by various feeds versus a control meal with poultry meat on cardiovascular risk markers and plasma protein expression are investigated. The ...

The Effects of Trout Fed With a Vegetable Based Feed on Cardiovascular Risk Markers and Plasma Proteome

Fish from the aquaculture sector constitutes an increasing part of the fish consumption in Denmark. The most important farmed fish in Denmark is the rainbow trout. Due to limited access of...

Effects of SNP of GnRH Receptor Genes in IVF Patients

Gonadotropin-releasing hormone analogue (GnRH-a) "long protocol" is a protocol for pituitary down-regulation in IVF. However, it is common in clinic that some patients are hypersensitive t...

Sense2Stop:Mobile Sensor Data to Knowledge

The aim of this research is to build systems that can recognize when people are stressed and then provide them with relaxation prompts in the moment to reduce their likelihood of being str...

Hypoglycemia Associated Autonomic Failure in Type 1 Diabetes Mellitus

The purpose of this study is to determine what corticosteroid receptor (and the dose of) is responsible for cortisol inducing hypoglycemia associated autonomic dysfunction in Type 1 DM. S...

Medical and Biotech [MESH] Definitions

A species of gram-negative bacteria responsible for red mouth disease in rainbow trout (ONCORHYNCHUS MYKISS). The bacteria is a natural component of fresh water ecosystems in the United States and Canada.

A species of gram-negative bacteria responsible for red mouth disease in rainbow trout (ONCORHYNCHUS MYKISS). The bacteria is a natural component of fresh water ecosystems in the United States and Canada.

A large stout-bodied, sometimes anadromous, TROUT found in still and flowing waters of the Pacific coast from southern California to Alaska. It has a greenish back, a whitish belly, and pink, red, or lavender stripes on the sides, with usually a sprinkling of black dots. It is highly regarded as a sport and food fish. Its former name was Salmo gairdneri. The sea-run rainbow trouts are often called steelheads. Redband trouts refer to interior populations of rainbows.

A DNA-binding orphan nuclear receptor that negatively regulates expression of ARNTL TRANSCRIPTION FACTORS and plays a role as a regulatory component of the circadian clock system. The Nr1d1 nuclear receptor expression is cyclically-regulated by a feedback loop involving its positive regulation by CLOCK PROTEIN; BMAL1 PROTEIN heterodimers and its negative regulation by CRYPTOCHROME and PERIOD PROTEINS.

A signal transducing tumor necrosis factor receptor associated factor that is involved in TNF RECEPTOR feedback regulation. It is similar in structure and appears to work in conjunction with TNF RECEPTOR-ASSOCIATED FACTOR 1 to inhibit APOPTOSIS.

Quick Search


DeepDyve research library

Relevant Topic

Mental Health
Adhd Anorexia Depression Dyslexia Mental Health Psychiatry Schizophrenia Stress Mental health, although not being as obvious as physical health, is very important, causing great unhappiness to those affected, causing add...

Searches Linking to this Article