Anti-FIRΔexon2, a splicing variant form of PUF60, auto-antibody is detected in the sera of esophageal squamous cell carcinoma.

08:00 EDT 13th April 2019 | BioPortfolio

Summary of "Anti-FIRΔexon2, a splicing variant form of PUF60, auto-antibody is detected in the sera of esophageal squamous cell carcinoma."

Anti-PUF60 autoantibodies are reportedly detected in the sera of patients with dermatomyositis and Sjogren's syndrome; however, little is known regarding its existence in the sera of cancer patients. FIR, a splicing variant of the PUF60 gene, is a transcriptional repressor of c-myc. In colorectal cancer, there is an overexpression of the dominant negative form of FIR, in which exon 2 is lacking (FIRΔexon2). Previously, large-scale SEREX (serological identification of antigens by recombinant cDNA expression cloning) screenings have identified anti-FIR auto-antibodies in the sera of cancer patients. In the present study, we revealed the presence and significance of anti-FIR (FIR/FIRΔexon2) antibodies (Abs) in the sera of patients with esophageal squamous cell carcinoma (ESCC). Our results were validated by performing an Alpha-LISA (Amplified Luminescence Proximity Homogeneous Assay) using sera of patients with various cancers types. We revealed that anti-FIRΔexon2 Ab had higher sensitivity than anti-FIR Ab. ROC (receiver operating curve) analysis was performed for evaluating the use of anti-FIRΔexon2 Ab as candidate markers such as anti-p53 antibody (anti-p53 Ab) and CEA, and the highest area under the ROC analysis was observed in combination of anti-FIRΔexon2 Ab and anti-p53 Ab. In summary, our results suggest the use of anti-FIRΔexon2 Ab in combination with the anti-p53 Ab as a predictive marker for ESCC. AUC (area under the curve) value was further increased in the advanced stage of ESCC. A novel clinical indicator against of ESCC by anti-FIRΔexon2 auto-antibody as purposing a companion diagnostics is discussed. This article is protected by copyright. All rights reserved.


Journal Details

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Name: Cancer science
ISSN: 1349-7006


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