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Adenovirus is a kind of non-enveloped,double-stranded DNA virus. As a member of the mammalian adenoviruses of the Adenoviridae family, porcine adenovirus causes gastrointestinal disease in piglets. In this study, the modified adenovirus was manipulated to carry a green fluorescence EGFP marker. The modified adenovirus was added to medium199 for co-incubation or microinjected into the cytoplasm of porcine oocytes. The effect of adenovirus on the first polar body extrusion was not significant during porcine oocyte maturation. Our data demonstrated the zona pellucida plays a vital role in porcine oocytes being resistant to modified adenovirus. Additionally, the results suggested that oocytes protect themselves from nonself substances.
This article was published in the following journal.
The purpose of this study was to determine whether the mitochondrial membrane potential, pro-apoptotic gene expression, and ubiquitylation status of zona pellucida proteins (ZP1, ZP2, and ZP3) of vitr...
Bovine herpesvirus 1 (BHV1) is an important bovine pathogen, responsible for respiratory diseases and reproductive problems. This study investigated the penetration capacity of BHV1 into oocytes after...
[This corrects the article DOI: 10.1371/journal.pone.0218963.].
Our purpose was to study whether application of femtosecond laser pulses for alphanumeric code marking in the volume of zona pellucida (ZP) could be effective and reliable approach for direct tagging ...
Zona pellucida (ZP), which enwraps the oocyte during folliculogenesis, initially forms in the primary follicle and plays an important role in female fertility. Here, we investigated a mouse strain ("m...
A defect in the hatching stage is considered an important cause of implantation failure. Therefore, assisted hatching (AH), which involves artificial disruption of the zona pellucida, has ...
The aim of study is to assess the possible impact of assisted hatching on delivery rate after transfer of vitrified-warmed human blastocysts.
Investigators aim to investigate the effect of elimination of blastocoelic fluid by creating a large hole in the zona pellucida at the cellular junction of the trophectoderm cells located ...
In vitro fertilization (IVF) treatment is now commonly used to treat infertile couples. During IVF treatment, oocytes and sperm are routinely incubated overnight and this may lead to subop...
Several studies indicate that Nitric Oxide (NO) plays an important role in the physiology of the reproductive system in mammals. It has been shown that NO affects sperm motility, it regula...
Membrane glycoproteins that constitute the ZONA PELLUCIDA in mammals and function in the recognition and binding of SPERMATOZOA.
A tough transparent membrane surrounding the OVUM. It is penetrated by the sperm during FERTILIZATION.
Female germ cells derived from OOGONIA and termed OOCYTES when they enter MEIOSIS. The primary oocytes begin meiosis but are arrested at the diplotene state until OVULATION at PUBERTY to give rise to haploid secondary oocytes or ova (OVUM).
The very first viral gene products synthesized after cells are infected with adenovirus. The E1 region of the genome has been divided into two major transcriptional units, E1A and E1B, each expressing proteins of the same name (ADENOVIRUS E1A PROTEINS and ADENOVIRUS E1B PROTEINS).
Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...