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The main aim of the present review is to present the opportunities and challenges associated with the application of cryopreserved sperm, which may improve the breeding of salmonid fishes. Cryopreservation of sperm has been used as a strategy for the conservation of biodiversity of fishes populations, the preservation of sperm from the most valuable breeding individuals and facilitate transportation of genomes, and providing a biological source of sperm regardless of the synchronisation of the maturity of broodstocks. Cryopreserved sperm can be used for the genetic improvement of salmonid fishes based on the programs of individual crossing of selected males with individual females. However, these opportunities have not yet been fully implemented at the conditions of hatchery practice. Despite the significant progress concerning the standardization of cryopreservation procedures, there are still more challenges than opportunities related to the implementation of sperm cryopreservation into breeding of salmonid fishes. The main challenge concerns the scaling up of the method towards fulfilling the requirements of fishes-breeders, in particular mass production of eyed eggs and fry. The present review shows knowledge gaps that should be considered in further studies, including development of methods to obtain sufficient amounts of sperm from numerous species of salmonids, scaling up the methods towards cryopreservation of high volumes of sperm and towards thawing high number of straws, and optimizing artificial fertilization in which oocytes are fertilized with high numbers of frozen/thawed sperm. Moreover, the implementation of technologies into hatchery practice will require special consideration to counteract the risk of sperm infection and its transmission to offsprings during cryopreservation and storage in liquid nitrogen.
This article was published in the following journal.
Sperm vitrification (V) is a method for cryopreservation, without the use of conventional cryoprotectants, by plunging the sperm suspension directly into liquid nitrogen (LN25).
New genome-editing tools, such as ZFNs, TALEN, and CRISPR/Cas9, have enabled the generation of gene-modified models effectively in mammals. These technologies are a powerful tool for studying gene fun...
Gamete cryopreservation causes cellular damage and death. This study develops cryopreservation techniques for Levantine scraper, and deciphers how early offspring development is affected when eggs are...
Cryopreservation is a technique that can keep sperm alive indefinitely, enabling the conservation of male fertility. It involves the cooling of semen samples and their storage at -196 °C in liquid n...
The objective is to systematically review and synthesize the literature on the efficacy with two different cryopreservation methods used for human spermatozoa and evaluate whether vitrification protoc...
Sperm freezing has been employed for decades for male fertility preservation in cases of foreseeable or unexpected loss of fertility to guarantee future paternity, and also as a complement...
Cryopreservation is the storage of biological material at subzero temperatures at which biochemical processes of cell metabolism and the biochemical reactions that lead to cell death are s...
The objective of this study is to evaluate the effect of in-vitro myoinositol supplementation of human sperm on the outcome of cryopreservation.
Despite the known adverse effects of specific cancer treatments on fertility, only 18-26% of at-risk adolescents and young men cryopreserve sperm prior to cancer treatment in the US:These ...
Although it is widely used, slow freezing can induce strong functional and nuclear spermatic alterations reducing the chances of pregnancy. The study objective is to determinate the effect...
An assisted fertilization technique consisting of the microinjection of a single viable sperm into an extracted ovum. It is used principally to overcome low sperm count, low sperm motility, inability of sperm to penetrate the egg, or other conditions related to male infertility (INFERTILITY, MALE).
The quality of SEMEN, an indicator of male fertility, can be determined by semen volume, pH, sperm concentration (SPERM COUNT), total sperm number, sperm viability, sperm vigor (SPERM MOTILITY), normal sperm morphology, ACROSOME integrity, and the concentration of WHITE BLOOD CELLS.
Preservation of cells, tissues, organs, or embryos by freezing. In histological preparations, cryopreservation or cryofixation is used to maintain the existing form, structure, and chemical composition of all the constituent elements of the specimens.
Interactive processes between the oocyte (OVUM) and the sperm (SPERMATOZOA) including sperm adhesion, ACROSOME REACTION, sperm penetration of the ZONA PELLUCIDA, and events leading to FERTILIZATION.
A method of providing future reproductive opportunities before a medical treatment with known risk of loss of fertility. Typically reproductive organs or tissues (e.g., sperm, egg, embryos and ovarian or testicular tissues) are cryopreserved for future use before the medical treatment (e.g., chemotherapy, radiation) begins.
Biological therapy involves the use of living organisms, substances derived from living organisms, or laboratory-produced versions of such substances to treat disease. Some biological therapies for cancer use vaccines or bacteria to stimulate the body&rs...
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