Track topics on Twitter Track topics that are important to you
Azo dyes are an important group of recalcitrant xenobiotics, which are difficult to degrade and deteriorate in cold environments. In this study, two microbial consortia consisting of cold-adapted and mesophilic bacteria were developed for effective decolorization of Reactive Black-5 azo dye. These bacteria were isolated from textile wastewater and soil of a cold region. Identification of bacterial isolates using 16s rRNA gene analysis revealed that they belong to genus Pseudoarthrobacter, Gordonia, Stenotrophomonas, and Sphingomonas. Decolorization assay was performed for every strain at dye concentrations of 25, 50 and 100 mg/L and the consortia PsGo consisting of mesophilic bacteria and StSp consisting of cold-adapted bacteria were constructed accordingly. Results showed that the consortia PsGo and StSp were able to decolorize 54 and 34 percent of RB-5 (50 mg/L) during 7 days. To improve the dye removal efficiency of the consortia, several parameters including temperature, pH, carbon and nitrogen sources were optimized. Over longer periods, StSp consortium managed to completely decolorize RB-5 (50 mg/L) at optimized conditions of 25-30 °C, pH 9, and using glucose and NHHPO as carbon and nitrogen source respectively, whereas PsGo consortium decolorized RB-5 (50 mg/mL) completely at 37 °C, pH 11, and with lactose and NHHPO used as carbon and nitrogen sources. Kinetic of reactions for StSp and PsGo consortia were found to be 0.05 and 0.13 day respectively, but became 0.71 and 0.9 day after optimization. In general, cold ecosystems are good sources for the isolation of novel bacterial strains with a potential application, especially when used as consortia, in environmental biotechnology such as decolorization of RB-5.
This article was published in the following journal.
Name: Journal of environmental management
Bioelectrochemical systems (BESs) have shown great potential for azo dye removal. However, comprehensive evaluation of the bioelectrochemical decolorization performance for reactive diazo dyes remains...
DNA ligases operating at low temperatures have potential advantages for use in biotechnological applications. For this reason, we have characterized the temperature optima and thermal stabilities of t...
Laccases are used in decolorization and biodegradation of synthetic dyes, bioremediation of industrial wastewaters and delignification of lignocellulosic compounds. The aims of the present study were ...
Effluents loaded with various synthetic dyes are considered as a huge burden to the surrounding ecosystems. Sudan dyes are relatively difficult to decolorize due to its water-insolubility. In the pres...
The aim of this work was to study the ability of commercial immobilized hydrolases in the decolorization of molasses wastewater. Commercial immobilized keratinase obtained the highest decolorization y...
This is a single center, prospective, randomized controlled study. The primary objective of this study is to measure migration over two years with RSA. Patients will be randomized in two a...
The optimization of recovery to alleviate the effects of fatigue in athletes can provide valuable performance advantage. However, despite the growing body of literature regarding effects o...
Complete resection of neoplastic polyps is pivotal, as 8.8% to 50% of interval cancers may arise as a consequence of incomplete polypectomy. However, the ideal method to remove small color...
The study will compare the use of cold snare, hot snare, cold EMR, and hot EMR for polyp resection. Although previous studies have compared two of the potential resection methods, no previ...
The objective of this study is to demonstrate that frequent atrio-ventricular (AV/PV) and inter-ventricular (V-V) delay optimization using QuickOpt in patients with CRT-D device results in...
Bacteria that can survive and grow in the complete, or nearly complete absence of oxygen.
A constellation of responses that occur when an organism is exposed to excessive cold. In humans, a fall in skin temperature triggers gasping, hypertension, and hyperventilation.
A condition characterized by the recurrence of HEMOGLOBINURIA caused by intravascular HEMOLYSIS. In cases occurring upon cold exposure (paroxysmal cold hemoglobinuria), usually after infections, there is a circulating antibody which is also a cold hemolysin. In cases occurring during or after sleep (paroxysmal nocturnal hemoglobinuria), the clonal hematopoietic stem cells exhibit a global deficiency of cell membrane proteins.
A climate characterized by COLD TEMPERATURE for a majority of the time during the year.
A form of therapy consisting in the local or general use of cold. The selective destruction of tissue by extreme cold or freezing is CRYOSURGERY. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)
Bioinformatics is the application of computer software and hardware to the management of biological data to create useful information. Computers are used to gather, store, analyze and integrate biological and genetic information which can then be applied...
An assay is an analytic procedure for qualitatively assessing or quantitatively measuring the presence or amount or the functional activity of a target entity. This can be a drug or biochemical substance or a cell in an organism or organic sample. ...
Antibodies Antisense Assays Biochips Bioinformatics Biological Therapy Biomarkers Biomaterials Bioscience Cell Culture Cloning Cytokine Diagnostics Dna Extraction Dna Sequencing Dna Transform...