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The objective of this research was to apply near-infrared spectroscopy, with a short-wavelength range of 950 to 1,650 nm, for the rapid detection of aflatoxin B (AFB) contamination in polished rice samples. Spectra were obtained by reflection mode for 105 rice samples: 90 samples naturally contaminated with AFB and 15 samples artificially contaminated with AFB. Quantitative calibration models to detect AFB were developed using the original and pretreated absorbance spectra in conjunction with partial least squares regression with prediction testing and full cross-validation. The statistical model from the external validation process developed from the treated spectra (standard normal variate and detrending) was most accurate for prediction, with a correlation coefficient ( r) of 0.952, a standard error of prediction of 3.362 μg/kg, and a bias of -0.778 μg/kg. The most predictive models according to full cross-validation were developed from the multiplicative scatter correction pretreated spectra ( r = 0.967, root mean square error in cross-validation [RMSECV] = 2.689 μg/kg, bias = 0.015 μg/kg) and standard normal variate pretreated spectra ( r = 0.966, RMSECV = 2.691 μg/kg, bias = 0.008 μg/kg). A classification-based partial least squares discriminant analysis model of AFB contamination classified the samples with 90% accuracy. The results indicate that the near-infrared spectroscopy technique is potentially useful for screening polished rice samples for AFB contamination.
This article was published in the following journal.
Name: Journal of food protection
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Furano-furano-benzopyrans that are produced by ASPERGILLUS from STERIGMATOCYSTIN. They are structurally related to COUMARINS and easily oxidized to an epoxide form to become ALKYLATING AGENTS. Members of the group include AFLATOXIN B1; aflatoxin B2, aflatoxin G1, aflatoxin G2; AFLATOXIN M1; and aflatoxin M2.
A noninvasive technique that uses the differential absorption properties of hemoglobin and myoglobin to evaluate tissue oxygenation and indirectly can measure regional hemodynamics and blood flow. Near-infrared light (NIR) can propagate through tissues and at particular wavelengths is differentially absorbed by oxygenated vs. deoxygenated forms of hemoglobin and myoglobin. Illumination of intact tissue with NIR allows qualitative assessment of changes in the tissue concentration of these molecules. The analysis is also used to determine body composition.
A potent hepatotoxic and hepatocarcinogenic mycotoxin produced by the Aspergillus flavus group of fungi. It is also mutagenic, teratogenic, and causes immunosuppression in animals. It is found as a contaminant in peanuts, cottonseed meal, corn, and other grains. The mycotoxin requires epoxidation to aflatoxin B1 2,3-oxide for activation. Microsomal monooxygenases biotransform the toxin to the less toxic metabolites aflatoxin M1 and Q1.
Magnetic resonance spectroscopy involving detection of changes in the resonance of carbon-13 nuclei in organic molecules.
A spectroscopic technique in which a range of wavelengths is presented simultaneously with an interferometer and the spectrum is mathematically derived from the pattern thus obtained.
Food is any substance consumed to provide nutritional support for the body. It is usually of plant or animal origin, and contains essential nutrients, such as carbohydrates, fats, proteins, vitamins, or minerals. The substance is ingested by an organism ...