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Optomechanical Time-Gated Fluorescence Imaging Using Long-Lived Silicon Quantum Dot Nanoparticles.

08:00 EDT 15th April 2019 | BioPortfolio

Summary of "Optomechanical Time-Gated Fluorescence Imaging Using Long-Lived Silicon Quantum Dot Nanoparticles."

We demonstrate a novel optomechanical synchronization method to achieve ultrahigh-contrast time-gated fluorescence imaging using live zebrafish as models. Silicon quantum dot nanoparticles (SiQDNPs) with photoluminescence lifetime of about 16 µs are used as the long-lived probes to enable background autofluorescence removal and multiplexing through time-gating. A continuous-wave 405-nm laser, as the excitation source, is focused on a rotating optical chopper, on which the emission light beam obtained from an inverted fluorescence microscope is also focused but with a phase difference, such that in a short delay after the excitation laser is blocked, the emission light beam passes through the optical chopper, initiating the image acquisition by a conventional sensor. Both excitation and detection time windows are synchronized by one optical chopper, eliminating the need for pulsed light source and image intensifier which is often used as ultrafast optical shutter. By using the cost-effective time-gating method, nearly all background autofluorescence emitted from the yolk sac of a zebrafish embryo microinjected with the SiQDNPs was removed, leading to a 45-fold increase in signal-to-background ratio. Furthermore, two kinds of fluorescence signals emitted from the microinjected SiQDNPs and the intrinsic green fluorescent protein of transgenic zebrafish larvae can be clearly separated through time-gating.

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Name: Analytical chemistry
ISSN: 1520-6882
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