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Heavy-atom labeling of RNA by PLOR for de novo crystallographic phasing.

08:00 EDT 15th April 2019 | BioPortfolio

Summary of "Heavy-atom labeling of RNA by PLOR for de novo crystallographic phasing."

Due to the paucity of known RNA structures, experimental phasing is crucial for obtaining three-dimensional structures of RNAs by X-ray crystallography. Covalent attachment of heavy atoms to RNAs is one of the most useful strategies to facilitate phase determination. However, this approach is limited by the inefficiency or inability to synthesize large RNAs (>60 nucleotides) site-specifically labeled with heavy atoms using traditional methods. Here, we applied our recently reported method, PLOR (position-selective labeling of RNA) to incorporate 5-iodouridine at specific positions in the adenine riboswitch RNA aptamer domain, which was then used for crystallization and subsequent de novo SAD phasing. PLOR is a powerful tool to improve the efficiency of obtaining RNA structures de novo by X-ray crystallography.

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This article was published in the following journal.

Name: PloS one
ISSN: 1932-6203
Pages: e0215555

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