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Due to the paucity of known RNA structures, experimental phasing is crucial for obtaining three-dimensional structures of RNAs by X-ray crystallography. Covalent attachment of heavy atoms to RNAs is one of the most useful strategies to facilitate phase determination. However, this approach is limited by the inefficiency or inability to synthesize large RNAs (>60 nucleotides) site-specifically labeled with heavy atoms using traditional methods. Here, we applied our recently reported method, PLOR (position-selective labeling of RNA) to incorporate 5-iodouridine at specific positions in the adenine riboswitch RNA aptamer domain, which was then used for crystallization and subsequent de novo SAD phasing. PLOR is a powerful tool to improve the efficiency of obtaining RNA structures de novo by X-ray crystallography.
This article was published in the following journal.
Name: PloS one
We present a novel crystallographic screening methodology (MiniFrags) that employs high-concentration aqueous soaks with a chemically diverse and ultra-low-molecular-weight library (heavy atom count 5...
Since heavy halogen atoms can be used to tune the emissive properties of organic luminogens, the understanding of their role in the photophysics is fundamental for materials engineering. Here, the ext...
Protein dynamics is pivotal to biological processes. However, experiments are very demanding and difficult to perform, and all-atom molecular dynamics simulations can still not provide all the answers...
Previously unknown the steric heavy atom effect on magnetic anisotropy parameters of triplet phenyl nitrenes is reported. The heavy bromine atom effect is revealed by W-band EPR and theoretical invest...
In a pilot study, heavy water labeling was used to determine HBsAg turnover rates in chronic hepatitis B (CHB) patients. The mean (SD) half-life of HBsAg in blood was 6.7 (5.5) days which therefore re...
This interventional study investigates the efficacy of Wet cupping therapy on heavy metal levels.The levels of the selected heavy metals will be measured in the blood samples obtained from...
Heavy menstrual bleeding or menorrhagia, is a common problem on women's lives and can burden both patients and health care systems. HMB is defined as cyclic heavy vaginal bleeding. Hormona...
This study is to assess the utility of high resolution 3D echo planar magnetic resonance spectroscopy (3D EPSI) in monitoring Novo-TTF response in glioblastoma multiforme (GBM) patients.
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The purpose of this study is to examine the effects of quetiapine in reducing percent heavy drinking days and increasing percent abstinent days in alcohol dependent patients who are freque...
Techniques for labeling a substance with a stable or radioactive isotope. It is not used for articles involving labeled substances unless the methods of labeling are substantively discussed. Tracers that may be labeled include chemical substances, cells, or microorganisms.
Nuclear reaction in which the nucleus of a heavy atom such as uranium or plutonium is split into two approximately equal parts by a neutron, charged particle, or photon.
Carbon-containing thiophosphonic acid compounds. Included under this heading are compounds that have carbon bound to either SULFUR atom, PHOSPHOROUS atom, or the OXYGEN atom of the SPO2 core structure.
A group of beta-lactam antibiotics in which the sulfur atom in the thiazolidine ring of the penicillin molecule is replaced by a carbon atom. THIENAMYCINS are a subgroup of carbapenems which have a sulfur atom as the first constituent of the side chain.
The use of a heavy ion particle beam for radiotherapy, such as the HEAVY IONS of CARBON.
Radiology is the branch of medicine that studies imaging of the body; X-ray (basic, angiography, barium swallows), ultrasound, MRI, CT and PET. These imaging techniques can be used to diagnose, but also to treat a range of conditions, by allowing visuali...