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Cluster Formation and Entanglement in the Rheology of Antibody Solutions.

08:00 EDT 15th April 2019 | BioPortfolio

Summary of "Cluster Formation and Entanglement in the Rheology of Antibody Solutions."

Antibody solutions deviate from the dynamical and rheological response expected for globular proteins, especially as volume fraction is increased. Experimental evidence shows that antibodies can reversibly bind to each other via F and F domains, and form larger structures (clusters) of several antibodies. Here we present a microscopic equilibrium model to account for the distribution of cluster sizes. Antibody clusters are modeled as polymers that can grow via reversible bonds either between two F domains or between a F and a F. We propose that the dynamical and rheological behavior is determined by molecular entanglements of the clusters. This entanglement does not occur at low concentrations where antibody-antibody binding contributes to the viscosity by increasing the effective size of the particles. The model explains the observed shear-thinning behavior of antibody solutions.

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This article was published in the following journal.

Name: The journal of physical chemistry. B
ISSN: 1520-5207
Pages:

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Medical and Biotech [MESH] Definitions

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Sterile solutions, essentially free from foreign particles and suitably compounded and dispensed, for instillation into the eye. It does not include solutions for cleaning eyeglasses or CONTACT LENS SOLUTIONS. (From Dorland, 27th ed)

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