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Plant growth-promoting bacteria show promises in crop production, nevertheless, innovation in their stable delivery is required for practical use by farmers. Herein, the composite of poly(vinyl alcohol)/poly(vinylpyrrolidone) plasticized with glycerol and loaded with the microbial consortium (Bacillus subtilis plus Seratia marcescens) was fabricated and engineered onto canola (Brassica napus L.) seed via electrospinning. Scanning electron microscopy showed that biocomposite is a one-di-mensional membrane which encapsulated microbes in multilayered nanostructure and their interfacial behavior between microorganism and seed is beneficial for safer farming. Universal testing machine and thermogravimetric analysis demonstrated that biocomposite holds sufficient thermo-mechanical properties for stable handling and practical management. Spectroscopic study resolved the living hybrid-polymer structure of biocomposite and proved the plasticizing role of glycerol. Swelling study supports the degradation of biocomposite in the hydrophilic environment due to the leaching of plasticizer which is important for the sustained release of microbial cells. Shelf-life study supported that biocomposite seed-coat place threshold level of microbes (5.675 ± 0.48 log10 CFU/g)) and maintained their satisfactory viability for 15th-days at the room temperature. Antifungal and nutrient-solubilizing study supported that biocomposite seed-coat could provide opportunities to biocontrol diseases and improve nutrient acquisition by the plant. Pot study document the better performance of biocomposite seed-coat on seed germination, seedling growth, leaf area, plant dry biomass, and root system. Chemical and microbial study demonstrated that biocomposite seed-coat improved the effectiveness of bioinoculant in the root-soil interface where they survive, flourish and increase the nutrient pool status. In particular, this study present advances in the fabrication of biocomposite for encapsulation, preservation, sustained release and efficacious use of microorganisms onto seed for precision farming.
This article was published in the following journal.
Name: Journal of agricultural and food chemistry
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The encapsulated embryos of flowering plants. They are used as is or for animal feed because of the high content of concentrated nutrients like starches, proteins, and fats. Rapeseed, cottonseed, and sunflower seed are also produced for the oils (fats) they yield.
Vaccines in which the infectious microbial nucleic acid components have been destroyed by chemical or physical treatment (e.g., formalin, beta-propiolactone, gamma radiation) without affecting the antigenicity or immunogenicity of the viral coat or bacterial outer membrane proteins.
Enumeration by direct count of viable, isolated bacterial, archaeal, or fungal CELLS or SPORES capable of growth on solid CULTURE MEDIA. Each colony (i.e., microbial colony-forming unit) represents the progeny of a single cell in the original inoculum. The method is used routinely by environmental microbiologists for quantifying organisms in AIR; FOOD; and WATER; by clinicians for measuring patients' microbial load; and in antimicrobial drug testing.
ADP-RIBOSYLATION FACTOR 1 is involved in regulating intracellular transport by modulating the interaction of coat proteins with organelle membranes in the early secretory pathway. It is a component of COAT PROTEIN COMPLEX I. This enzyme was formerly listed as EC 18.104.22.168.
One or more types of plant seed proteins providing the large amounts of AMINO ACIDS utilized in GERMINATION and SEEDLING growth. As seeds are the major food source from AGRICULTURAL CROPS, seed storage proteins are a major source of DIETARY PROTEINS.
Mergers & Acquisitions
Commercial and market reports on mergers and acquisitions in the biotechnology, pharmaceutical, medical device and life-science industries. Mergers and acquisitions (abbreviated M&A;) is an aspect of corporate strategy, corporate finance and manageme...