Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry.

08:00 EDT 15th April 2019 | BioPortfolio

Summary of "Analysis of phenolic acids and flavonoids in leaves of Lycium barbarum from different habitats by ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry."

The leaves of Lycium barbarum (LLB) have been utilized as crude drugs and functional tea for human health in China and Southeast Asia for thousands of years. To control its quality, a rapid and sensitive ultra-high performance liquid chromatography coupled with triple quadrupole tandem mass spectrometry (UPLC-TQ-MS) method was established and validated for the first time for simultaneous determination of 10 phenolic acids and flavonoids (including neochlorogenic acid, protocatechuic aldehyde, p-hydroxybenzoic acid, chlorogenic acid, cryptochlorogenic acid, caffeic acid, p-coumaric acid, ferulic acid, rutin and kaempferol-3-O-rutinoside) in LLB. The separation was performed on an ACQUITY UPLC C chromatographic column (100 mm × 2.1 mm internal diameter, 1.7 μm particle size) with 0.1% formic acid in water (A) - acetonitrile (B) as the mobile phase under gradient elution. Multiple reaction monitoring (MRM) mode was adopted to simultaneously monitor the target components. The developed method was fully validated in terms of linearity (r ≥0.9860), precision (RSD≤6.58%), repeatability (RSD≤6.60%), stability (RSD≤6.17%), recovery (95.56%~108.06%, RSD≤4.64%) as well as limit of detection (0.021~0.664 ng/mL) and limit of quantity (0.069~2.210 ng/mL), and then successfully applied to evaluate the quality of 64 batches of LLB collected from 41 producing areas in 4 different provinces of China. The results showed that the LLB, especially collected from Inner Mongolia regions, were rich in the phenolic acids and flavonoids. Rutin, kaempferol-3-O-rutinoside and chlorogenic acid are the predominant compounds contained in LLB. The above findings will provide helpful information for the effective utilization of LLB.


Journal Details

This article was published in the following journal.

Name: Biomedical chromatography : BMC
ISSN: 1099-0801
Pages: e4552


DeepDyve research library

PubMed Articles [17050 Associated PubMed Articles listed on BioPortfolio]

Multielementar/centesimal composition and determination of bioactive phenolics in dried fruits and capsules containing Goji berries (Lycium barbarum L.).

The elemental/centesimal composition of dried fruits and capsules containing Goji berries (Lycium barbarum L.) was evaluated by ICP OES after two digestion procedures. Furthermore, phenolic acid compo...

Delay of cone degeneration in retinitis pigmentosa using a 12-month treatment with Lycium barbarum supplement.

Lycium barbarum L. (also known as "Goji berry"), a traditional Chinese herbal medicine, has been a common herb in the traditional Chinese pharmacopoeia for centuries. The main active component is the ...

LBP reduces theinflammatory injuryof kidney in septic rat and regulates the Keap1-Nrf2∕ARE signaling pathway1.

To investigate the influence of lycium barbarum polysaccharides (LBP), a functional derivative from lycium barbarum, on septic kidney injury.

Lycium barbarum polysaccharides grafted with doxorubicin: An efficient pH-responsive anticancer drug delivery system.

Lycium barbarum polysaccharide-doxorubicin (LBP-DOX) nanoparticles were synthesized via a one-step Schiff base reaction between oxidized Lycium barbarum polysaccharide (oxidized LBP) and doxorubicin (...

Lycium barbarum polysaccharide reduces hyperoxic acute lung injury in mice through Nrf2 pathway.

The disruption of the balance between antioxidants and oxidants plays a vital role in the pathogenesis of acute lung injury (ALI). Evidence has shown that Lycium barbarum polysaccharide (LBP) has anti...

Clinical Trials [2877 Associated Clinical Trials listed on BioPortfolio]

Effects of Vitamin C and Lycium Barbarum Supplements on the Progression of Senile Cataract in Hong Kong Chinese Population

With the rapid growing aging population, age-related eye diseases including cataract are becoming more prevalent. The demand for cataract surgery and post-surgical care posed enormous fina...

Goji Berries and Energy Expenditure

Effects of foods or food substances on energy expenditure are of particular interest, because of the increasing prevalence of obesity and its consequent metabolic diseases like type II dia...

Investigation on the Sustained Effect of Anthocyanins on Endothelial Function in Postmenopausal Women

Anthocyanins are the most common polyphenols in berries and red wine, along with other flavonoids, phenolic acids, minerals and vitamins. Anthocyanins are extensively metabolized and they ...

Oat Phenolics Bioavailability

A diet rich in whole grain is inversely associated with cardiovascular disease risk and this benefit could be partly attributed to the phenolic acid content of whole grains. The exact abso...

Effect of Phenolic Acids on the Human Vasculature

This study evaluates effects of different doses of phenolic acids on healthy volunteers.

Medical and Biotech [MESH] Definitions

A plant genus of the family OLEACEAE. Oleuropein, a phenolic secoiridoid glycoside, is found in the leaves.

A class of phenolic acids related to chlorogenic acid, p-coumaric acid, vanillic acid, etc., which are found in plant tissues. It is involved in plant growth regulation.

A plant genus of the family SOLANACEAE. Members contain CEREBROSIDES and SCOPOLETIN.

A collective name for a group of closely related lipids that contain substitutions on the 2H-1-benzopyran-6-ol nucleus and a long hydrocarbon chain of isoprenoid units. They are antioxidants by virtue of the phenolic hydrogen. Tocopherols react with the most reactive form of oxygen and protect unsaturated fatty acids from oxidation.

A plant genus of the family ANACARDIACEAE. This is the source of the familiar cashew nuts, which are heat treated to remove the irritant toxin. Cashew nut shell liquid (frequently abbreviated as CNSL) is a major source of alkenyl phenolic compounds, especially ANACARDIC ACIDS, cardol, and cardanol.

Quick Search


DeepDyve research library

Relevant Topic

Multiple Sclerosis MS
Multiple sclerosis (MS) is the most common disabling neurological condition affecting 100,000 young adults in the UK. The condition results from autoimmune damage to myelin, causing interference in nerve signaling. Symptoms experienced depend on the pa...

Searches Linking to this Article