Advertisement

Topics

Highly-sensitive UHPLC-DAD method for simultaneous determination of two synergistically-acting antiepileptic drugs: Application to pharmaceutical tablets and human urine.

08:00 EDT 15th April 2019 | BioPortfolio

Summary of "Highly-sensitive UHPLC-DAD method for simultaneous determination of two synergistically-acting antiepileptic drugs: Application to pharmaceutical tablets and human urine."

A simple and highly sensitive Ultra High-performance Liquid Chromatographic -Diode array detection method (UHPLC-DAD) was developed and validated for the simultaneous estimation of levetiracetam (LEV) and lacosamide (LAC). It was clinically proven that the combination of LEV and LAC exhibits a synergistic effect against refractory seizures in mice, which was the motivation for the analysis of this binary mixture both in bulk and human urine samples. The binary mixture was resolved on a Hypersil BDS C analytical column, utilizing a mobile phase of 0.050 mol L phosphate buffer (pH 5.60), methanol and acetonitrile in the ratio (80:10:10 v/v/v) using catechol as an internal standard (IS). The mobile phase was pumped at a flow rate of 1.2 mL min with diode array detection (DAD) at 205 nm for both drugs and 270 nm for IS. Calibration curves were linear with correlation coefficient (> 0.9990) over the studied concentration range of 0.1 - 70.0 μg mL for both drugs. The developed method was found to be reproducible with low relative standard deviation (RSD) values for intra- and inter-day precision (< 2.0 %). Both drugs were determined in bulk, pharmaceutical formulations, and human urine samples without any interference from complex matrices.

Affiliation

Journal Details

This article was published in the following journal.

Name: Biomedical chromatography : BMC
ISSN: 1099-0801
Pages: e4554

Links

DeepDyve research library

PubMed Articles [19285 Associated PubMed Articles listed on BioPortfolio]

Detection and quantification of 47 antibiotic residues in farmed European sea bass (Dicentrarchus labrax) using a multi-class and multi-residue UHPLC-MS/MS method.

In the present study, a multi-class multi-residue method for the simultaneous detection and determination of antibiotics in European sea bass (Dicentrarchus labrax) was developed and validated. The me...

An accurate and reproducible method for simultaneous determination of four flavonoids in EtOAc extracts from Sophora flavescens Ait. in rat plasma based on UHPLC Q-Exactive Mass spectrometry: application to a pharmacokinetics study.

In previous studies, it was revealed that ethyl acetate (EtOAc) extracts from Sophora flavescens Ait. improved glucose tolerance, reduced hyperglycemia, and restored insulin levels to treat diabetes. ...

Simultaneous determination of 58 pesticides and relevant metabolites in eggs with a multi-functional filter by ultra-high performance liquid chromatography-tandem mass spectrometry.

A sensitive method for the simultaneous determination of 58 pesticides and relevant metabolites in eggs was developed using ultra-high performance liquid chromatography-tandem mass spectrometry (UHPLC...

A rapid and sensitive UHPLC-MS/MS method for the determination of celosin A in rat plasma with application to pharmacokinetic study.

Celosin A (CA), a natural compound isolated from Celosia argentea L., has been shown significant hepatoprotective effect on AHNP-induced liver injury. This study described a rapid and sensitive ultra ...

Simultaneous determination of FLZ and its metabolite (M1) in human plasma and urine by UHPLC-MS/MS: Application to a pharmacokinetic study.

FLZ is a novel anti-Parkinson's disease candidate drug. The main active metabolite is FLZ O-dealkylation (M1) in preclinical studies. A reliable ultra-high-performance liquid chromatography-tandem mas...

Clinical Trials [6016 Associated Clinical Trials listed on BioPortfolio]

An Evaluation of IV Gamma Globulin As a Method to Improve Kidney Transplant Survival in Patients With End-Stage Renal Disease Who Are Highly Sensitized to Transplant Antigens

This study is designed to test the clinical and laboratory observations that suggest IVIG given before and after kidney transplant to patients who are sensitized (highly sensitive) to cert...

Evaluation of a Novel and Simplified Technique for Intrauterine Contraceptive Devices Insertion: a Prospective Cohort Study

The intrauterine devices (IUDs) are a safe, reliable, long-acting and reversible contraceptive method. It is also sound safe and cheap contraceptive methods available and is nearly mainten...

Safety and Efficacy of Simultaneous or Staged Surgery on Complicated Hepatolithiasis

For some complicated hepatolithiasis, it is difficult to decide whether to perform surgery on biliary stone and portal hypertension simultaneously or separately by staged surgery.There's a...

BBOT: Bacterial Burden in Ortho Trauma Procedures

The purpose of this research project is to improve understanding of the potential role of highly sensitive bacterial tests in diagnosing infected non-healing fractures compared to the curr...

Evaluation of a Novel and Simplified Technique for Intrauterine Contraceptive Devices Insertion

The intrauterine devices are a safe, reliable, long-acting and reversible contraceptive method. It is also sound safe and cheap contraceptive methods available and is nearly maintenance fr...

Medical and Biotech [MESH] Definitions

A highly-sensitive (in the picomolar range, which is 10,000-fold more sensitive than conventional electrophoresis) and efficient technique that allows separation of PROTEINS; NUCLEIC ACIDS; and CARBOHYDRATES. (Segen, Dictionary of Modern Medicine, 1992)

The simultaneous, or near simultaneous, transference of heart and lungs from one human or animal to another.

A set of techniques used when variation in several variables has to be studied simultaneously. In statistics, multivariate analysis is interpreted as any analytic method that allows simultaneous study of two or more dependent variables.

A mercaptocholine used as a reagent for the determination of CHOLINESTERASES. It also serves as a highly selective nerve stain.

Simultaneous infection of a host organism by two or more pathogens. In virology, coinfection commonly refers to simultaneous infection of a single cell by two or more different viruses.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topics

Urology
Benign Prostatic Hyperplasia (BPH) Erectile Dysfunction Urology Urology is the branch of medicine concerned with the urinary tract and diseases that affect it. Examples include urethritis, urethrostenosis and incontinence. Urology is a su...

Diagnostics
A diagnostic test is any kind of medical test performed to aid in the diagnosis or detection of disease. For example: to diagnose diseases to measure the progress or recovery from disease to confirm that a person is free from disease Clin...


Searches Linking to this Article