Mitochondria-Targeting Intracellular Delivery of Native Proteins by Using Biodegradable Silica Nanoparticles.

08:00 EDT 17th April 2019 | BioPortfolio

Summary of "Mitochondria-Targeting Intracellular Delivery of Native Proteins by Using Biodegradable Silica Nanoparticles."

Mitochondria are key organelles in mammalian cells whose dysfunctions are linked to various diseases. Drugs targeting mitochondrial proteins thus provide a highly promising strategy for potential therapeutics. Current methods of mitochondria-targeting delivery of small molecule drugs are available, but not suitable for macromolecules such as proteins (including antibodies). Herein, we report the first example of mitochondria-targeting intracellular delivery of native proteins (and antibodies) by using biodegradable silica nanoparticles (BS-NPs). Endowed with surface-modified triphenylphosphonium (TPP) and cell-penetrating poly(disulfide)s (CPD), these protein-loaded nanocapsules were capable of rapid intracellular uptake with minimal endolysosomal trapping, thus providing sufficient time for effective mitochondrial localization followed by glutathione-triggered biodegradation and mitochondrial release of native functional proteins to take part in further biological processes.


Journal Details

This article was published in the following journal.

Name: Angewandte Chemie (International ed. in English)
ISSN: 1521-3773


DeepDyve research library

PubMed Articles [16121 Associated PubMed Articles listed on BioPortfolio]

Direct Drug Targeting into Intracellular Compartments: Issues, Limitations, and Future Outlook.

Intracellular compartment drug delivery is a promising strategy for the treatment of diseases. By this way, medicines can delivered to particular intracellular compartments. This maximizes the therape...

Synthetic biology approaches for targeted protein degradation.

Protein degradation is an effective native mechanism used in modulating intracellular information, and thus it plays an essential role in maintaining cellular homeostasis. Repurposing native protein d...

Regulation of targeting determinants in interorganelle communication.

The field of interorganelle communication is now established as a major aspect of intracellular organisation, with a profusion of material and signals exchanged between organelles. One way to address ...

Tag and release: strategies for the intracellular cleavage of protein conjugates.

Attaching a functional moiety to a protein is required for a wealth of applications, comprising targeted delivery, controlling of enzyme activity, and prodrug-based therapy. Targeting intracellular pr...

A thermoresponsive nanocarrier for mitochondria-targeted drug delivery.

Mitochondria emerge as an important target for cancer therapy. Herein, by taking advantage of the recently reported high temperature of mitochondria, a well-tuned thermoresponsive nanocarrier was deve...

Clinical Trials [4980 Associated Clinical Trials listed on BioPortfolio]

Clinical Application of Autologous Mitochondria Transplantation for Improving Oocyte Quality.

Embryo quality was ranked as one of the most important predictors in determining the success of implantation, while clinically some patients may experience repeated IVF failure due to pers...

Evaluation of the Use of a Biodegradable Endoprothesis in the Prevention of Post-Endoscopic Retrograde CholoangioPancreatography Pancreatitis

The use of an endoprothesis in a pancreatic duct is a measure to minimize the incidence and severity of Post-Endoscopic Retrograde CholoangioPancreatography Pancreatitis. In this study we ...

Mitochondria Inborn Errors of Metabolism and ANT Defects in Mitochondria Diseases

The objective of this research protocol is to continue investigation of the nature and prevalence of mitochondria disease and to aid patients and health care providers in the understanding...

Biodegradable Stenting Anastomoses Versus Double-layer Hand Sutures for Reconstruction in Intestinal Anastomosis

Intestinal anastomosis plays an important role in various general surgeries, but the complications such as anastomotic leakage,stenosis and hemorrhage cannot been avoided. Although many wa...

Protective Effect of Biodegradable Collagen Implant (Ologen) in Scleral Thinning After Strabismus Surgery

This study is to investigate protective effect of biodegradable collagen implant in scleral showing after lateral rectus recession in patients with exotropia.

Medical and Biotech [MESH] Definitions

Protein domains of approximately 120 amino acids that form two perpendicular anti-parallel beta sheets connected by a loop of variable length and a C-terminal amphipathic helix. PH domains occur in many INTRACELLULAR SIGNALING PROTEINS and CYTOSKELETAL PROTEINS where they bind PHOSPHATIDYLINOSITOLS within the CELL MEMBRANE and INTRACELLULAR MEMBRANES, as well as proteins that include the Betagamma-subunits of HETEROTRIMERIC GTP-BINDING PROTEINS, and PROTEIN KINASE C. These interactions allow the targeting of proteins to different cellular compartments and SIGNAL TRANSDUCTION PATHWAYS.

Systems for the delivery of drugs to target sites of pharmacological actions. Technologies employed include those concerning drug preparation, route of administration, site targeting, metabolism, and toxicity.

Forms to which substances are incorporated to improve the delivery and the effectiveness of drugs. Drug carriers are used in drug-delivery systems such as the controlled-release technology to prolong in vivo drug actions, decrease drug metabolism, and reduce drug toxicity. Carriers are also used in designs to increase the effectiveness of drug delivery to the target sites of pharmacological actions. Liposomes, albumin microspheres, soluble synthetic polymers, DNA complexes, protein-drug conjugates, and carrier erythrocytes among others have been employed as biodegradable drug carriers.

Mitochondria of skeletal and smooth muscle. It does not include myocardial mitochondria for which MITOCHONDRIA, HEART is available.

Mitochondria in hepatocytes. As in all mitochondria, there are an outer membrane and an inner membrane, together creating two separate mitochondrial compartments: the internal matrix space and a much narrower intermembrane space. In the liver mitochondrion, an estimated 67% of the total mitochondrial proteins is located in the matrix. (From Alberts et al., Molecular Biology of the Cell, 2d ed, p343-4)

Quick Search

DeepDyve research library

Relevant Topics

An antibody is a protein produced by the body's immune system when it detects harmful substances, called antigens. Examples of antigens include microorganisms (such as bacteria, fungi, parasites, and viruses) and chemicals. Antibodies may be produc...

Within medicine, nutrition (the study of food and the effect of its components on the body) has many different roles. Appropriate nutrition can help prevent certain diseases, or treat others. In critically ill patients, artificial feeding by tubes need t...

Searches Linking to this Article