Reactivity of Platelet-activating and Non-platelet-activating anti-PF4/Heparin Antibodies in Enzyme Immunosorbent Assays under different Conditions.

08:00 EDT 22nd April 2019 | BioPortfolio

Summary of "Reactivity of Platelet-activating and Non-platelet-activating anti-PF4/Heparin Antibodies in Enzyme Immunosorbent Assays under different Conditions."

Enzyme immunosorbent assays (EIA) are widely used to detect human anti-platelet factor 4/heparin antibodies (aPF4/H Abs) to rule out heparin-induced thrombocytopenia. However, EIAs cannot differentiate between clinically relevant, platelet-activating and non-relevant, non-platelet activating Abs and only ~50% of patients' sera testing positive by EIA contain antibodies which activate platelets. Recently, we have shown platelet activating aPF4/H Abs bind more strongly to PF4/H complexes than non-platelet activating antibodies. Antigen-antibody interactions are known to depend on electrostatic interactions governed by pH, heat, and ionic strength. We tested, whether changes in pH and ionic strength can improve the specificity of EIAs detecting aPF4/H Abs.


Journal Details

This article was published in the following journal.

Name: Journal of thrombosis and haemostasis : JTH
ISSN: 1538-7836


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