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Midazolam (MDZ) is the first choice in palliative sedation, and commonly used in sleep induction in anesthesia, with rapid onset of action. However, monitoring the level of sedation in patients does not occur accurately. We developed and validated a bioanalytical method to detect MDZ in plasma using high performance liquid chromatography (HPLC) coupled to a photodiode array detector (PDA) for future monitoring of sedation. MDZ was extracted by solid phase extraction (SPE). Analyses were performed on a C18 column, using 0.05% triethylamine and acetonitrile as mobile phase, analyzing at 220 nm. Recovery was evaluated by comparing extracted and non-extracted solutions. Precision, accuracy, linearity, limits of detection (LD) and quantification (LQ), specificity and selectivity were determined. The mean recovery obtained by SPE extraction was 101.03%. The method was linear in the range 1.0 - 50.0 μg/mL. LD and LQ are, respectively, 0.43 and 1.43 μg/mL. The specificity of MDZ peak presented an adequate purity. The method was able to detect the drug MDZ among other drugs. Plasma anticoagulants showed no interference in the drug detection. The bioanalytical method using HPLC-PDA and SPE was successfully validated and showed linearity, precision, accuracy, specificity and presents high sensitivity for detection of MDZ in human plasma.
This article was published in the following journal.
Name: Biomedical chromatography : BMC
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