Advertisement

Topics

A Versatile Macro-Based Neurohistological Image Analysis Suite for ImageJ Focused on Automated and Standardized User Interaction and Reproducible Data Output.

08:00 EDT 4th May 2019 | BioPortfolio

Summary of "A Versatile Macro-Based Neurohistological Image Analysis Suite for ImageJ Focused on Automated and Standardized User Interaction and Reproducible Data Output."

In recent decades, the advent of advanced microscopy techniques, including high resolution digital imaging, multi-dimensional acquisition, and multiple fluorescence channel exposure, as well as the availability of inexpensive terabyte-capacity digital storage, has enabled neuroscience research laboratories to engage in high-throughput quantitative image analysis experiments involving numerous chemical markers and experimental conditions covering multiple brain regions and composed of hundreds of micrographs. Analyzing and processing these large data sets presents challenges in ensuring precision and reproducibility under demanding time and training constraints.

Affiliation

Journal Details

This article was published in the following journal.

Name: Journal of neuroscience methods
ISSN: 1872-678X
Pages:

Links

DeepDyve research library

PubMed Articles [30056 Associated PubMed Articles listed on BioPortfolio]

Automated Image Acquisition and Morphological Analysis of Cell Growth Mutants in Physcomitrella patens.

This protocol describes the automated imaging and a quantitative analysis of the morphology of small plants from the moss Physcomitrella patens. This method can be used for the analysis of growth phen...

The use of ROI overlays and a semi-automated method for measuring cortical area in ImageJ for histological analysis.

A major part of histologic studies is the use of high resolution imaging for data collection and analysis. ImageJ, a freely available software from the NIH designed for image analysis, has many featur...

MyelinJ: An ImageJ macro for high throughput analysis of myelinating cultures.

MyelinJ is a free user friendly ImageJ macro for high throughput analysis of fluorescent micrographs such as 2D-myelinating cultures and statistical analysis using R. MyelinJ can analyse single images...

Image Analysis: Basic Procedures for Description of Plant Structures.

This chapter gives examples of basic procedures of quantification of plant structures with use of image analysis, which are commonly employed to describe differences among experimental treatments or p...

An ImageJ tool for simplified post-treatment of TEM phase contrast images (SPCI).

Tools for taking advantage of phase-contrast in transmission electron microscopy are of great interest for both biological and material sciences studies as shown by the recent use of phase plates and ...

Clinical Trials [6563 Associated Clinical Trials listed on BioPortfolio]

Negative Pressure Wound Therapy: Are All Systems Alike?

Hypotheses: (1)Wound healing during hospital stay will be equal between systems, (2)RNs will perceive the Versatile One (EZCare) system as easier to use, (3)Costs related to dressing chang...

The Multifunctional Image Guided Therapy Suite (MIGTS) in Emergency Multiple Trauma Care

Controlled clinical intervention study comparing the treatment of multiple trauma patients in a newly developed combined diagnostic and operation room, the multifunctional image guided the...

Image and Model Based Analysis of Lung Disease

The purpose of this research study is to develop CT scanning and image analysis techniques to help define and measure several key properties of the pulmonary system that cannot be obtained...

Chronic Indwelling Foley and Catheter Associated Trauma (CIF-CAT) Study

To determine whether an indwelling low profile Kohli Atraumatic Catheter causes less macro-morphologic inflammation and edema to the bladder mucosa than a traditional indwelling Foley cath...

Cataract Refractive Suite Study

The purpose of this study is to compare the refractive predictability (prediction error) between the Cataract Refractive Suite (CRS) and standard manual technique at one month post-operati...

Medical and Biotech [MESH] Definitions

A method of chemical analysis based on the detection of characteristic radionuclides following a nuclear bombardment. It is also known as radioactivity analysis. (McGraw-Hill Dictionary of Scientific and Technical Terms, 4th ed)

A technique encompassing morphometry, densitometry, neural networks, and expert systems that has numerous clinical and research applications and is particularly useful in anatomic pathology for the study of malignant lesions. The most common current application of image cytometry is for DNA analysis, followed by quantitation of immunohistochemical staining.

A method of delineating blood vessels by subtracting a tissue background image from an image of tissue plus intravascular contrast material that attenuates the X-ray photons. The background image is determined from a digitized image taken a few moments before injection of the contrast material. The resulting angiogram is a high-contrast image of the vessel. This subtraction technique allows extraction of a high-intensity signal from the superimposed background information. The image is thus the result of the differential absorption of X-rays by different tissues.

Analysis based on the mathematical function first formulated by Jean-Baptiste-Joseph Fourier in 1807. The function, known as the Fourier transform, describes the sinusoidal pattern of any fluctuating pattern in the physical world in terms of its amplitude and its phase. It has broad applications in biomedicine, e.g., analysis of the x-ray crystallography data pivotal in identifying the double helical nature of DNA and in analysis of other molecules, including viruses, and the modified back-projection algorithm universally used in computerized tomography imaging, etc. (From Segen, The Dictionary of Modern Medicine, 1992)

The production of an image obtained by cameras that detect the radioactive emissions of an injected radionuclide as it has distributed differentially throughout tissues in the body. The image obtained from a moving detector is called a scan, while the image obtained from a stationary camera device is called a scintiphotograph.

Advertisement
Quick Search
Advertisement
Advertisement

 


DeepDyve research library

Relevant Topic

Mergers & Acquisitions
Commercial and market reports on mergers and acquisitions in the biotechnology, pharmaceutical, medical device and life-science industries. Mergers and acquisitions (abbreviated M&A;) is an aspect of corporate strategy, corporate finance and manageme...


Searches Linking to this Article