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Orthogonal Engineering of Biosynthetic Pathway for Efficient Production of Limonene in Saccharomyces cerevisiae.

08:00 EDT 7th May 2019 | BioPortfolio

Summary of "Orthogonal Engineering of Biosynthetic Pathway for Efficient Production of Limonene in Saccharomyces cerevisiae."

Limonene, a plant-derived natural cyclic monoterpene, is widely used in pharmaceutical, food and cosmetics industries. Conventional limonene biosynthetic (CLB) pathway in engineered Saccharomyces cerevisiae consists of heterologous limonene synthase (LS) using endogenous substrate GPP and has been suffering from poor production of limonene. In this study, we report on an orthogonal engineering strategy in S. cerevisiae for improving the production of limonene. We reconstructed orthogonal limonene biosynthetic (OLB) pathway composed of SlNDPS1 that catalyzes IPP and DMAPP to NPP ( cis-GPP) and plant LS that converts NPP to limonene. We find that the OLB pathway is more efficient for production of limonene than the CLB pathway. When expression of competing gene ERG20 was chromosomally regulated by glucose-sensing promoter HXT1, the OLB pathway harboring strain produced 917.7 mg/L of limonene in fed-batch fermentation, 6-fold increase of the CLB pathway, representing the highest titer reported to date. Orthogonal engineering exhibits great potential for production of terpenoids in S. cerevisiae.

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This article was published in the following journal.

Name: ACS synthetic biology
ISSN: 2161-5063
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